BOSS-RUNS for barcode balancing

goldman-gp-ebi / BOSS-RUNS

Dynamic, adaptive sampling during nanopore sequencing

GNU General Public License v3.0

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BOSS-RUNS for barcode balancing #6

Open ganschow opened 7 months ago

ganschow commented 7 months ago

Hi, Would it be possible to use BOSS-RUNS for barcode balancing with custom barcodes? Like specifying the barcodes (or adapter + barcode seqs) as targets and the tool will mitigate coverage bias between the barcoded samples?

W-L commented 6 months ago

Hi, Apologies for the very late reply! Unfortunately, this functionality is currently not implemented. Due to technical reasons reference sequences need to be of a certain minimum length (longer than typical nanopore reads). Otherwise internal calculations lead to unexpected results. For now, this is still on our todo list. Thanks and best wishes