google-deepmind / alphafold

Open source code for AlphaFold 2.
Apache License 2.0
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one error about alphafold,ask for help online #592

Open 285880830 opened 2 years ago

285880830 commented 2 years ago

CE8eSpRY.fasta 报错:I0910 11:42:53.106589 139985805367104 hhsearch.py:76] Launching subprocess "hhsearch -i /tmp/tmpd42j2lo8/query.a3m -o /tmp/tmpd42j2lo8/output.hhr -maxseq 1000000 -d /mnt/pdb70/pdb70" I0910 11:42:53.259523 139985805367104 utils.py:36] Started HHsearch query I0910 11:42:53.617978 139985805367104 utils.py:40] Finished HHsearch query in 0.358 seconds Traceback (most recent call last): File "run_alphafold.py", line 338, in app.run(main) File "/home/alphafold/miniconda3/envs/alphafold/lib/python3.8/site-packages/absl/app.py", line 312, in run _run_main(main, args) File "/home/alphafold/miniconda3/envs/alphafold/lib/python3.8/site-packages/absl/app.py", line 258, in _run_main sys.exit(main(argv)) File "run_alphafold.py", line 310, in main predict_structure( File "run_alphafold.py", line 170, in predict_structure feature_dict = data_pipeline.process( File "/home/alphafold/alphafold/alphafold/data/pipeline.py", line 166, in process hhsearch_result = self.hhsearch_pdb70_runner.query(uniref90_msa_as_a3m) File "/home/alphafold/alphafold/alphafold/data/tools/hhsearch.py", line 85, in query raise RuntimeError( RuntimeError: HHSearch failed: stdout:

stderr:

methylome commented 1 year ago

I got the same error.

yamule commented 1 year ago

11:42:53.447 WARNING: Ignoring invalid symbol '*' at pos. 1623 in line 2 of /tmp/tmpd42j2lo8/query.a3m

Don't you have '*' at the end of the query sequence?

DongyaoLiu commented 9 months ago

Yes, I have this at the end the protein, and I am facing identical error. I would run again without this one

amirh-hajianpour commented 8 months ago

OK! Unbelievably, the so-called "WARNING" is what causing the error! By just removing the asterisk symbol from the end of my sequence, my problem was resolved.