Closed fjptwenger closed 4 years ago
That seems to happen because there are no CDS features in the input gtf file (which looks like the output of a stringtie --merge operation, which only has exon
features but no CDS
features). Options -x
and -y
only produce output based on the CDS features.
I use command: gffread -x sample.CDS.fa -y sample.CDS.protein.fa -g GRCh38.fasta sample.gtf
and No errors appear,but both sample.CDS.fa and sample.CDS.protein.fa are empty file,Why is this happening?
the head of the sample.gtf: