When doing multiple comparisons of GFFs against the same reference file (even in the same gffcompare command) the number of Reference mRNAs: in the stats file keeps changing. I even tried to cluster the reference GFF beforehand but it seems impossible to pin these numbers down. Any tips?
Sorry, I found out the reason. For anyone else searching it's the -R option. Removing it makes the number of reference transcripts consistent between all comparisons.
Hi,
gffread: 0.12.7 gffcompare: 0.12.6
When doing multiple comparisons of GFFs against the same reference file (even in the same gffcompare command) the number of
Reference mRNAs:
in the stats file keeps changing. I even tried to cluster the reference GFF beforehand but it seems impossible to pin these numbers down. Any tips?