gpertea / stringtie

Transcript assembly and quantification for RNA-Seq
MIT License
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prepDE.py error: sample file not generated by -e option #360

Closed zjj19910 closed 2 years ago

zjj19910 commented 2 years ago

I assembled the transcripts using stringtie and re-generated the quantification file using the merged gtf file.

cat ../accessionlist | while read id; do echo "stringtie ../${alignment_dir}/${id}.sorted.bam -p 24 -Be -G stringtie_merged.gtf -o ${id}/${id}.stringtie_merged.gtf -A ${id}.gene_abund_merged.tab"; done |sh

But when I was preparing the count matrix using prepDE.py, an error occurred named

prepDE.py -i sample_list.txt -g gene_count_matrix.csv -t transcript_count_matrix.csv

Error: sample file ./HL-PBS-1/HL-PBS-1.stringtie_merged.gtf was not generated with -e option!

while it was indeed generated from the -e option, how did this error occur? Thank you!

lgg84 commented 1 year ago

me to.Have you solved it yet?

CYH-BI commented 1 year ago

me to.have anyone solved this problem,I used -e before but I still reported this error when using prepDE.py later on.thanks