Closed zjj19910 closed 2 years ago
I assembled the transcripts using stringtie and re-generated the quantification file using the merged gtf file.
cat ../accessionlist | while read id; do echo "stringtie ../${alignment_dir}/${id}.sorted.bam -p 24 -Be -G stringtie_merged.gtf -o ${id}/${id}.stringtie_merged.gtf -A ${id}.gene_abund_merged.tab"; done |sh
But when I was preparing the count matrix using prepDE.py, an error occurred named
prepDE.py -i sample_list.txt -g gene_count_matrix.csv -t transcript_count_matrix.csv
Error: sample file ./HL-PBS-1/HL-PBS-1.stringtie_merged.gtf was not generated with -e option!
while it was indeed generated from the -e option, how did this error occur? Thank you!
me to.Have you solved it yet?
me to.have anyone solved this problem,I used -e before but I still reported this error when using prepDE.py later on.thanks
I assembled the transcripts using stringtie and re-generated the quantification file using the merged gtf file.
But when I was preparing the count matrix using prepDE.py, an error occurred named
Error: sample file ./HL-PBS-1/HL-PBS-1.stringtie_merged.gtf was not generated with -e option!
while it was indeed generated from the -e option, how did this error occur? Thank you!