gpertea / stringtie

Transcript assembly and quantification for RNA-Seq
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Segmentation Error with Stringtie mix. The bundle appears too large. #395

Open niradsp opened 1 year ago

niradsp commented 1 year ago

I have close to 21 samples and all of them are giving me a segmentation error message. I ran one of them in -v mode, and here is what I see: [04/03 02:07:28]>bundle chr12:62620768-67427718 [207329419 alignments (2957891 distinct), 31713 junctions, 93 guides] begins processing...

Isn't this bundle too big?
This is monkey data. I ran short-read using STAR, and long-read using minimap2 with -ax splice:hq and -uf option (the PacBio data is after the refine step). The BAM files are both sorted and indexed.

Any suggestions?

Thanks in advance, Nirad