gpertea / stringtie

Transcript assembly and quantification for RNA-Seq
MIT License
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How do I deal with this warning message? #414

Open Kwon419 opened 10 months ago

Kwon419 commented 10 months ago

Warning: gene " TRNAK-UUU " (on NC_022272.1) has reference transcripts on both strands?

Hellow,

I Googled and found out that this warning message comes out when there are both positive and negative strands in the reference gtf file.

I got a warning message but there seems to be no problem with the reference I used. Download ref link: https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000364345.1/

I am curious about two things.

First, Can I ignore the warning massage and proceed?

A warning message came up, but I got a gene count table as output.

I'm curious about the penalty of ignoring the warning message.

Second, I wonder how to solve the warning message.

If anyone knows about this, please answer.