I'm using stringtie:2.2.2 to assemble transcripts by --mix model. All samples were finished successfully except one sample. and I separated the bam file by chromosome to check the bam file followed by https://github.com/gpertea/stringtie/issues/227 .
I found the error was reported again in the process of chr6 assembly. Here is the command:
stringtie -o mammary.gland_4_mix_sample_REF_NC_037333.1.out.gtf --mix -p 1 -G GCF_002263795.3_ARS-UCD2.0_genomic.gff mammary.gland_4Aligned.sortedByCoord.out.REF_NC_037333.1.bam mammary.gland_4.sorted.REF_NC_037333.1.bam
Please let me know if you have any ideas about what's causing this and what I can do next.
Hello,
I'm using stringtie:2.2.2 to assemble transcripts by --mix model. All samples were finished successfully except one sample. and I separated the bam file by chromosome to check the bam file followed by https://github.com/gpertea/stringtie/issues/227 .
I found the error was reported again in the process of chr6 assembly. Here is the command:
stringtie -o mammary.gland_4_mix_sample_REF_NC_037333.1.out.gtf --mix -p 1 -G GCF_002263795.3_ARS-UCD2.0_genomic.gff mammary.gland_4Aligned.sortedByCoord.out.REF_NC_037333.1.bam mammary.gland_4.sorted.REF_NC_037333.1.bamPlease let me know if you have any ideas about what's causing this and what I can do next.
Best wishes,
Qi