Hi, when I assemble transcripts by StringTie. I found there are a lot of single exon transcripts without strand information. But when I run other software by the newly transcriptome assembly gtf file. It will report an error. So can I assign the strand information using xs or ts tag from bam files. Or remove them from gtf file?
Hi, when I assemble transcripts by StringTie. I found there are a lot of single exon transcripts without strand information. But when I run other software by the newly transcriptome assembly gtf file. It will report an error. So can I assign the strand information using xs or ts tag from bam files. Or remove them from gtf file?