Closed chloelulu closed 5 years ago
The description for each of the categories is found in the README here: https://github.com/grenaud/deML
Could you check the index for the ones for the different categories, there must be a reason why they get classified as such. Fail must be due to mismatches between the index templates and the sequenced indices.
have you had any luck with the README? I am just wondering if I can close this issue.
Hi, @grenaud ,
Thanks so much! The problem has been solved!
Hi, Thanks so much for creating this software. Just now I tried to demultiplex my data very quickly. But the output makes me confusing. Since many outputs I did not expected, shown in figure below. I only have one index.fq
Some fail.fq, some unknow.fq. which make me confusing.
The code I am using is
And my index file
src/deML -i index.txt -f sample_S0_L001_R1_001.fastq -r sample_S0_L001_R2_001.fastq -if1 sample_S0_L001_I1_001.fastq -o demultiplexed.
The format of my fq input files are shown in the picture below. May I know are there anything wrong? Thanks so much.