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grenaud / deML

Maximum likelihood demultiplexing
GNU General Public License v3.0
46 stars 13 forks source link

concerns on this software #8

Closed chloelulu closed 5 years ago

chloelulu commented 5 years ago

Hi, Thanks so much for creating this software. Just now I tried to demultiplex my data very quickly. But the output makes me confusing. Since many outputs I did not expected, shown in figure below. I only have one index.fq image Some fail.fq, some unknow.fq. which make me confusing. The code I am using is src/deML -i index.txt -f sample_S0_L001_R1_001.fastq -r sample_S0_L001_R2_001.fastq -if1 sample_S0_L001_I1_001.fastq -o demultiplexed. The format of my fq input files are shown in the picture below. May I know are there anything wrong? Thanks so much. image And my index file

image

grenaud commented 5 years ago

The description for each of the categories is found in the README here: https://github.com/grenaud/deML

Could you check the index for the ones for the different categories, there must be a reason why they get classified as such. Fail must be due to mismatches between the index templates and the sequenced indices.

grenaud commented 5 years ago

have you had any luck with the README? I am just wondering if I can close this issue.

chloelulu commented 5 years ago

Hi, @grenaud ,

Thanks so much! The problem has been solved!