Closed LiShuhang-gif closed 2 years ago
Hello! Yes the EPO was computed on hg19 not 38. I will write on my to-do list to try to find an EPO alignment that for hg38. Thank you for bringing this my attention.
Thank you for your reply! I am looking forward to your newly generated EPO file on hg38. By the way, I would like to ask how to obtain the required outgroup EPO file? Is it calculated by any software? Or which website can I download it from? Thanks!
Yes I parse them using https://github.com/grenaud/epoParser feel free to have a go at it if you want :-) The EPO for hg38 seems to be available here:http://www.ensembl.org/info/genome/compara/mlss.html?mlss=1993
Thanks again! I want to make sure whether the EPO files of chimpanzee downloaded from this website can directly be used in glactools? Or should I parse the EPO file of chimpanzee which I download from this ensembl website using epoParser first?
Not directly, you need to run it through epoParser which will produce a tab-delimited file.
Hi, I'm really sorry for the late reply due to my busy assessment. By the way, I was wondering if you generated an EPO file for hG38? Thanks!
Hey! I manage to find the EPO files on the ensemble FTP, I am parsing it now. Buzz me again next week.
Hey! I wonder if you have already found the EPO files on the ensemble FTP and generated an EPO file for hG38? Thanks in advance!
Yes! The files are here now:
ftp://ftp.healthtech.dtu.dk/public/EPO/all_hg19.epo.gz ftp://ftp.healthtech.dtu.dk/public/EPO/all_hg19.epo.gz.tbi ftp://ftp.healthtech.dtu.dk/public/EPO/all_hg38.epo.gz ftp://ftp.healthtech.dtu.dk/public/EPO/all_hg38.epo.gz.tbi
My apologies for the delay! I've also uploaded a new README with these links.
Let me know if you run into any trouble
Hi, I am currently trying to convert a VCF file to an ACF file using glactools. However, I have encountered the following error message:
I tried the solution in another issue. I've rewritten "chr22" as "22". However, this allowed some chromosomes to run smoothly, while others still reported errors.
I wonder if you have any suggestions for this? By the way, my variants were mapped to hg38 rather than hg19/37. Could this be the cause of the error message? Looking forward to your reply, thank you!