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grishkam / QuickFigures

QuickFigures is a toolset for creating scientific figures see publication for details
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Min/Max Adjustment of Channel #7

Open wuffi opened 1 year ago

wuffi commented 1 year ago

Dear Gregory, Thanks for developing this useful plugin and making it available to the community. I'm using the latest version of QuickFigures shipping with Fiji. Most parts of it work great, but setting whatever value in the "Min/Max" dialog opening upon right-click onto an image and choosing the "Channel" item in the pop-up list doesn't change the appearance of the image. Is there something I'm doing wrong?

grishkam commented 1 year ago

The min/max fields in the dialog are working on my computer. Perhaps you could share a screenshot and the type of image file you are using? That may help me understand what your issue is.

On Fri, Dec 16, 2022 at 11:29 AM wuffi @.***> wrote:

Dear Gregory, Thanks for developing this useful plugin and making it available to the community. I'm using the latest version of QuickFigures shipping with Fiji. Most parts of it work great, but setting whatever value in the "Min/Max" dialog opening upon right-click onto an image and choosing the "Channel" item in the pop-up list doesn't change the appearance of the image. Is there something I'm doing wrong?

— Reply to this email directly, view it on GitHub https://github.com/grishkam/QuickFigures/issues/7, or unsubscribe https://github.com/notifications/unsubscribe-auth/AEHSU4NOCNFKVBKSRF3PCOLWNSKGRANCNFSM6AAAAAATBGFAH4 . You are receiving this because you are subscribed to this thread.Message ID: @.***>

wuffi commented 1 year ago

Thanks for your answer, which helped me to pin down the problem: For ImageJ's classic "blob.gif" (8-bit) example, the dialog works as expected. If I take the "clown.jpg" example (RGB) example, there is no histogram in the dialog and changing the sliders to whatever values has no effect. Is the dialog only intended for monochrome (8-bit) images? Screenshot from 2022-12-17 12-08-48 Screenshot from 2022-12-17 12-07-33

grishkam commented 1 year ago

ImageJ has ways of converting RGB to 8-bit multichannel**. The dialog is not meant for RGB images. However, I recommend always saving files in your microscope's own format and opening/importing them into Fiji. That way, all colors and scales will be imported (those images will be 14-bit or 16bit multichannel images).

**If you must use RGB, you can go to "Image<Color<Split Channels" and then "Image<Color<Merge Channels..." in the ImageJ menubar to turn an RGB into a multichannel stack. I do not recommend this.

On Sat, Dec 17, 2022 at 6:15 AM wuffi @.***> wrote:

Thanks for your answer, which helped me to pin down the problem: For ImageJ's classic "blob.gif" (8-bit) example, the dialog works as expected. If I take the "clown.jpg" example (RGB) example, there is no histogram in the dialog and changing the sliders to whatever values has no effect. Is the dialog only intended for monochrome (8-bit) images? [image: Screenshot from 2022-12-17 12-08-48] https://user-images.githubusercontent.com/2758427/208239020-7e1c8592-2a6d-4811-9d99-76fee09a40da.png [image: Screenshot from 2022-12-17 12-07-33] https://user-images.githubusercontent.com/2758427/208239024-900f46bf-a6e0-4969-932d-da4cbd4f477a.png

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