grunwaldlab / audpc_example

An example analysis for calculating AUDPC using RMarkdown
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Update conclusions so it matches objectives of tutorial #4

Closed zkamvar closed 8 years ago

zkamvar commented 8 years ago

The current conclusions are not in line with the questions asked of the tutorial:

In general, the AUDPC only changes between the fungicide treatments during the last 10 days of the experiment. The "Cutter" seemed to be most resistant to the disease followed by "2137" and "Jagger". The results suggest that the best yields will be obtained when "Cutter" wheat is used and it is treated with fungicide.

Whereas the questions posed in the tutorial:

Additionally, it's not exactly correct to imply that Jagger has resistance, as it is noted as the "susceptible" variety:

The following example, based on field data from Kansas (Sparks and Stack, unpublished), illustrates the difference in disease severity for a variety susceptible to leaf rust, Jagger, and the resistant cultivars 2137 and Cutter.

Since we are going to be using this for a room full of plant pathologists, it would be a good idea to have this more accurate or their attention might be drawn towards nitpicking the analysis as opposed to the task at hand (creating the Rmd).

zachary-foster commented 8 years ago

I was not following the tutorial on the APS website, just using the dataset, but we can follow it if you want. Although, it seems to me that its best to make the content as simplistic as possible so that its obvious that the exercise is not about plant pathology but R markdown. The APS exercise is about plant pathology; ours is about Rmarkdown. Do you think we should do the exercise as described on the website?

I think of susceptibility and resistance to be on a continuum and not mutually exclusive. It seems like a simplification of reality to call one variety susceptible and the others resistant since they all get the disease, just to different degrees. "most resistant" and "least susceptible" seem the same to me, but I will rephrase it if you think it is an issue.

zkamvar commented 8 years ago

The APS exercise is about plant pathology; ours is about Rmarkdown. Do you think we should do the exercise as described on the website?

I think we should do the exercise as described for the simple fact that this will allow our audience to be more comfortable with the subject matter.

I think of susceptibility and resistance to be on a continuum and not mutually exclusive.

I think in this context, it's thought of as a positive control. While you can debate the merits of calling something "susceptible" vs "resistant", this is how it's thought of in plant pathology.

Ultimately, the idea is to present an exercise that our audience will be able to connect to. This is why I'm suggesting to do it the way the APS tutorial presents it. Students learn better if they can connect with the material. I do think it's an issue if we ignore the questions in the tutorial since they are relevant in the scope of plant pathology, which is where our audience is coming from.

zachary-foster commented 8 years ago

Ok, i will change it to more closely follow the exercise.

zachary-foster commented 8 years ago

This is done.

zkamvar commented 8 years ago

In general, the AUDPC only changes between the fungicide treatments during the last 20 days of the experiment.

It's a bit more accurate to say that the disease progress curve changes, not the AUDPC

zachary-foster commented 8 years ago

Good point, I will change it.

zachary-foster commented 8 years ago

I think this is done.