Hi,
I am using the following command to run Panaroo and getting a file extension error. I annotate the genome using prokka. Besides that, I tried using the off file from NCBI but still got the error.
Panaroo requires multiple GFF files to build a pangenome. As you are supplying only one (Ent-11.gff), it is assuming the file should be a list of paths to other GFFs which is causing the error.
Hi, I am using the following command to run Panaroo and getting a file extension error. I annotate the genome using prokka. Besides that, I tried using the off file from NCBI but still got the error.
panaroo -i Ent-11.gff -o entomo-prokka -a core --core_threshold 0.90 --merge_paralogs --clean-mode strict --aligner mafft --remove-invalid-genes -t 40
Thank you.