Open sandratra-rab opened 1 month ago
Warning! We cannot directly obtain demultiplexed pod5, we need to 1) basecall 2) demultiplex 3) extract read IDs from demultiplexed reads 4) use pod5 tools to get demultiplexed pod5
We can only get demultiplexed UBAM and FASTQ files
STORAGE ISSUES ? It could be a bottleneck if we need to re-basecall right after minknow in order to store the demultiplexed pod5 files
Solutions:
Add barcodes management/demultiplexing option when performing basecalling