I am trying to apply GangSTR on exome sequencing samples. However, I encountered an issue about 'Not enough reads extracted' across all the TR regions. Could you help me to figure it out? May I know how many reads are essential for a given TR region? (I can image the scenario as the figure1 in your NAR paper) The commands I used are listed below. Thank you in advance!
Hello GangSTR team,
I am trying to apply GangSTR on exome sequencing samples. However, I encountered an issue about 'Not enough reads extracted' across all the TR regions. Could you help me to figure it out? May I know how many reads are essential for a given TR region? (I can image the scenario as the figure1 in your NAR paper) The commands I used are listed below. Thank you in advance!
Calculate coverage for the given cram file
Calculate the average coverage
Calculate the
insertmean
andinsertsdev
using samtoolsRun GangSTR
Output errors
Meanwhile, the output VCF is empty with only header lines.