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hammerlab / flowdec

TensorFlow Deconvolution for Microscopy Data
Apache License 2.0
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PSF min max and type. #36

Closed joaomamede closed 3 years ago

joaomamede commented 3 years ago

Should the PSF be Float32 with min being 0 and max being 1?

Can I use an acquired PSF straight from a 16bit image? Or should I contrast from 0 to 1?

Thanks.

eric-czech commented 3 years ago

All you need for a PSF is non-negative values. It would be converted to float32 (by default) and rescaled to sum to 1, but you don't need to do either of those things beforehand.

joaomamede commented 3 years ago

That's awesome! I'm having great results with flowdec. It already deconvolved 22TB of data :) Thanks again