The problem seems to be in the plot-gating-template chunk in Flow.Rmd. The book builds fine if I comment out this chunk and everything below it.
Here's the masking caused by that cell:
Adding population:Live
Adding population:Lymphocytes
Adding population:Singlets
Adding population:gdTCells
Adding population:abTCells
Adding population:maiTCells
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:dplyr':
combine, intersect, setdiff, union
The following object is masked from 'package:fs':
path
The following object is masked from 'package:flowCore':
normalize
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
anyDuplicated, append, as.data.frame, basename, cbind,
colMeans, colnames, colSums, dirname, do.call, duplicated,
eval, evalq, Filter, Find, get, grep, grepl, intersect,
is.unsorted, lapply, lengths, Map, mapply, match, mget, order,
paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind,
Reduce, rowMeans, rownames, rowSums, sapply, setdiff, sort,
table, tapply, union, unique, unsplit, which, which.max,
which.min
Attaching package: 'graph'
The following object is masked from 'package:stringr':
boundary
The error I get when this chunk is left uncommented:
label: pgse2770tidy (with options)
List of 1
$ dependson: chr "pgse2770"
Quitting from lines 325-331 (learning-about-t-cells-through-data.Rmd)
Error in mutate_impl(.data, dots) :
Evaluation error: argument "graph" is missing, with no default.
Calls: <Anonymous> ... <Anonymous> -> mutate -> mutate.tbl_df -> mutate_impl
In addition: There were 49 warnings (use warnings() to see them)
Execution halted
Exited with status 1.
The problem seems to be in the
plot-gating-template
chunk inFlow.Rmd
. The book builds fine if I comment out this chunk and everything below it.Here's the masking caused by that cell:
The error I get when this chunk is left uncommented: