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hammerlab
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t-cell-guide
Human Primary T cells: A Practical Guide
Apache License 2.0
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Mention StemCell's Direct T cell isolation kit option
#27
armish
opened
5 years ago
0
Document the immunofluorescence protocol
#26
armish
opened
5 years ago
1
Add link to protocols.io
#25
hammer
closed
5 years ago
2
See which one of the Yamanaka factors improves GFP mRNA expression
#24
armish
closed
5 years ago
1
Explore the effect of Tregs on IL2-dependent proliferation
#23
armish
opened
6 years ago
0
Explored long-term effect of using fewer activation beads
#22
armish
opened
6 years ago
0
Add electroporation-mediated cell death data as a notebook
#21
armish
opened
6 years ago
1
add links to the individual notebooks from the README
#20
armish
closed
6 years ago
0
Add T/PBMC count data from 4 new donors and update the T/PBMC boxplot
#19
armish
closed
6 years ago
0
Optimization of mRNA electroporation in naive T cells
#18
armish
closed
6 years ago
0
Cas9 electroporation in RNP format (CD4 KO)
#17
armish
closed
6 years ago
0
add GFP mRNA electroporation data
#16
armish
closed
6 years ago
0
Cytokine profiling
#15
hammer
opened
6 years ago
2
add flow data on IL2 titration (Sigma vs NIH)
#14
armish
closed
6 years ago
0
Add counts from the IL2 titration (NIH vs Sigma)
#13
armish
closed
6 years ago
0
minor update to the PBMC figure
#12
armish
closed
6 years ago
0
add PBMC -> T cell counts
#11
armish
closed
6 years ago
0
Add baseline T subset frequencies
#10
armish
closed
6 years ago
0
Add IL2 titration results
#9
armish
closed
6 years ago
0
Add resazurin and activation bead titration data
#8
armish
closed
6 years ago
0
Add cell counts from pre- and post-activation cultures across six donors
#7
armish
closed
6 years ago
0
add direct PBMC activation data
#6
armish
closed
6 years ago
0
Add isolation bead titration data
#5
armish
closed
6 years ago
0
Put all the protocols on Protocols.io and figures on Figshare
#4
armish
closed
6 years ago
1
Mention alternatives to Neon and Nucleofector for non-viral transfection
#3
hammer
opened
6 years ago
0
Mention alternatives to Dynabeads for activation and expansion
#2
hammer
opened
6 years ago
0
Define the scope of this guide
#1
hammer
closed
6 years ago
1