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hammerlab / tcr-transfer

Explore an alternative way to clone TILs by first single-cell TCR-seq'ing them and then transforming non-exhausted T cells with these TIL TCRs
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De-orphanize MC38B TCRs #8

Open armish opened 4 years ago

armish commented 4 years ago

Given that we have some preliminary data on TCRs being MC38B reactive, we would like to explore their respective targets. For this, we need to

  1. get the cell line sequenced against our B6 host (as normal) (#5)
  2. knock out either H2Kb or H2Db and test these variants against our TCRs to see if either of them will eliminate the cytotoxicity (#6)
  3. try previously described MC38 tetramers on the cells (#7)
  4. adopt a DNA-library-based presentation system (e.g. PresentER) to screen for hundreds of candidates
hammer commented 4 years ago

A few months ago I had to review this topic and put my notes up on Discourse. I need to update that topic with the new work that has landed in the past few months but I'd be happy to share what I've learned if we get past step 3.