hannekeo
commented
3 years ago Hi Sveta Thanks for alerting me to this issue. It appears to be an issue that FIJI has with the order of line_width * 0.8 as I have reversed it and the macro works now. You have two options:
- Either you correct line 55 -like I did- into:
@.***D74753.E661B660]
- Or you download the new version that I have just uploaded to GitHub
You may also find that the generation of the mask does not work optimally in your images in which case you can tweak this part in the macro to work better on your data (i.e. lines 196-200). This is only relevant if you wanted to use the masks in CellProfiler to quantify intensity signals, and not for generation of montages.
Worm_align in the first instance was meant to just be a simple C.Elegans montage maker, but “morphed” into a way to do analysis as well. Many thanks for using Worm_align! It is great to see my macro being used, and I hope it is helpful in your research.
Kind regards Hanneke
From: svetmymirror @.> Sent: 12 May 2021 16:43 To: hannekeo/Worm-align @.> Cc: Subscribed @.***> Subject: [hannekeo/Worm-align] Macro Error (#1)
Hi there,
I am stuck at the step of identifying the width of the worm, as when I click OK, Fiji gives me "macro error", saying: ";" expected in line 55 l = line_width <*> 0.8 ;
How could I resolve this?
Best, Sveta
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHubhttps://url6.mailanyone.net/v1/?m=1lgr1K-0008K2-4B&i=57e1b682&c=a3Gu1rSkkYgc78MrHRx2jjyDXOOGymfdqB6LciK66LxofiUGwVDk5SmrtFI1n4gu9M6MQ3sIb166xpZ7RXkqbKe0JV1CInDLagVNe-cVqjntexmySOjyksGMARxthjPwil6X_ZbqWnZRFhh-7nvrjg8muloTp1Xo-cjG_h1Cnw5S3inU33WD1WpZe76S2V22v5owP48bT3FzKmTOlrGsk69_DftddkiDXJQn3kjpRh_ywRRLxUIEbDes7AZvNKuO, or unsubscribehttps://url6.mailanyone.net/v1/?m=1lgr1K-0008K2-4B&i=57e1b682&c=L-mu0HbHgrKtwlXVaw4u2H24GBZqom_iDER09tIp68E_DQJcnO0zZ20MCFEWQhBwVXlaTGQgJve1w0xlJs8P-jJW4ZRN67nEVCaOCGvcPthyFvIyw8rDr-eBkDEV9Cs7hlFWpT2_bxmeHDVcYt3lVtEONA7phDX8ghrHvJg15X7Vx1MiVcPA9ScJ2OVQFLg7nuIsHyD7OK7UvWRmxr-yrXS5_2_uBpdSYmGiO8TJUAV3w6pik2IkG8n3cXAdFdcnTwGi3TK-Tqh4fUnWW6t8Xdr5uithOsGv4O5Inx8Cr83LbhASyMb9LeFR2pH29TJf.
hello-lei
berk1835
Hi there,
I am stuck at the step of identifying the width of the worm, as when I click OK, Fiji gives me "macro error", saying: ";" expected in line 55 l = line_width <*> 0.8 ;
How could I resolve this?
Best, Sveta