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hansenjn / CiliaQ

An ImageJ plugin to quantify ciliary shape, length, and fluorescence.
GNU General Public License v3.0
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Skeleton image - basal body representation is off #15

Open hansenjn opened 3 months ago

hansenjn commented 3 months ago

In the newest version, CiliaQ places the basal body marking in the Skeleton image in a place remote from the beginning of the skeleton. There must be some coordinate conversion problem when writing the basal body mark into the skeleton image.

image

hansenjn commented 3 months ago

Update: This problem only appears when the image calibration settings are 1 voxel in x,y, and z. If they are specified in micron, in the same image, all looks fine (see below): image