hansenjn / CiliaQ

An ImageJ plugin to quantify ciliary shape, length, and fluorescence.
GNU General Public License v3.0
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CiliaQ produces empty output file #3

Closed sRassmann closed 4 years ago

sRassmann commented 4 years ago

CiliaQ constantly produces empty output txt and no skeleton file for certain file (provided privately).

hansenjn commented 4 years ago

This issue was not originating from CiliaQ but from the configuration of the file. The file was supposed to be handled as a "3D stack" with multiple slices and one timepoint but was erroneously defined as a "timelapse 2D stack" with one slice and multiple timepoints. In ImageJ, this is visualized at the bars below the image:

As a note: For timelapse stacks, CiliaQ only outputs time-averages for a cilium in the main output file, if the cilium is present in all time frames.