hansenlab / minfi

Devel repository for minfi
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FlowSorted.Blood.EPIC install? #94

Closed pc2001 closed 7 years ago

pc2001 commented 7 years ago

Hi, I was trying to use minfi to estimate cell counts for illumina EPIC data. I upgraded both R and bioconductor, but couldn't execute estimateCellCounts because package "FlowSorted.Blood.EPIC" couldn't be loaded. Am I doing something wrong?

biocLite("FlowSorted.Blood.EPIC") BioC_mirror: https://bioconductor.org Using Bioconductor 3.4 (BiocInstaller 1.24.0), R 3.3.2 (2016-10-31). Installing package(s) ‘FlowSorted.Blood.EPIC’ Warning message: package ‘FlowSorted.Blood.EPIC’ is not available (for R version 3.3.2) sessionInfo() R version 3.3.2 (2016-10-31) Platform: x86_64-apple-darwin13.4.0 (64-bit) Running under: OS X El Capitan 10.11.4

locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages: [1] grid stats4 parallel stats graphics grDevices utils datasets methods
[10] base

other attached packages: [1] FlowSorted.Blood.450k_1.12.0 gap_1.1-16
[3] som_0.3-5.1 pvclust_2.0-0
[5] reshape_0.8.6 data.table_1.10.2
[7] foreign_0.8-67 preprocessCore_1.36.0
[9] gtools_3.5.0 RnBeads_1.6.1
[11] plyr_1.8.4 methylumi_2.20.0
[13] minfi_1.20.2 bumphunter_1.14.0
[15] locfit_1.5-9.1 iterators_1.0.8
[17] foreach_1.4.3 Biostrings_2.42.1
[19] XVector_0.14.0 SummarizedExperiment_1.4.0
[21] FDb.InfiniumMethylation.hg19_2.2.0 org.Hs.eg.db_3.4.0
[23] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2 GenomicFeatures_1.26.2
[25] AnnotationDbi_1.36.2 reshape2_1.4.2
[27] scales_0.4.1 Biobase_2.34.0
[29] illuminaio_0.16.0 matrixStats_0.51.0
[31] limma_3.30.9 gridExtra_2.2.1
[33] gplots_3.0.1 ggplot2_2.2.1
[35] fields_8.10 maps_3.1.1
[37] spam_1.4-0 ff_2.2-13
[39] bit_1.1-12 cluster_2.0.5
[41] RColorBrewer_1.1-2 MASS_7.3-45
[43] GenomicRanges_1.26.2 GenomeInfoDb_1.10.2
[45] IRanges_2.8.1 S4Vectors_0.12.1
[47] BiocGenerics_0.20.0 BiocInstaller_1.24.0

loaded via a namespace (and not attached): [1] nlme_3.1-130 bitops_1.0-6 httr_1.2.1
[4] tools_3.3.2 doRNG_1.6 nor1mix_1.2-2
[7] R6_2.2.0 KernSmooth_2.23-15 DBI_0.5-1
[10] lazyeval_0.2.0 colorspace_1.3-2 base64_2.0
[13] pkgmaker_0.22 rtracklayer_1.34.1 caTools_1.17.1
[16] genefilter_1.56.0 quadprog_1.5-5 stringr_1.1.0
[19] digest_0.6.12 Rsamtools_1.26.1 siggenes_1.48.0
[22] GEOquery_2.40.0 RSQLite_1.1-2 mclust_5.2.2
[25] BiocParallel_1.8.1 RCurl_1.95-4.8 magrittr_1.5
[28] Matrix_1.2-8 Rcpp_0.12.9 munsell_0.4.3
[31] stringi_1.1.2 zlibbioc_1.20.0 gdata_2.17.0
[34] lattice_0.20-34 splines_3.3.2 multtest_2.30.0
[37] annotate_1.52.1 beanplot_1.2 rngtools_1.2.4
[40] codetools_0.2-15 biomaRt_2.30.0 XML_3.98-1.5
[43] gtable_0.2.0 openssl_0.9.6 assertthat_0.1
[46] xtable_1.8-2 survival_2.40-1 tibble_1.2
[49] GenomicAlignments_1.10.0 registry_0.3 memoise_1.0.0

kasperdanielhansen commented 7 years ago

Yes.

You're trying to use reference data from EPIC arrays which is not available. Instead, use convertArray()to convert your data to look like 450k data and then run the function.