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harbourlab / SparK

Publication quality NGS track plotting
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Error using '-ps averages' #22

Open joebowness opened 1 year ago

joebowness commented 1 year ago

Hello!

Thanks for making this tool! I have recently discovered it, and mostly it is working great for how I want to visualise my tracks.

The only thing I have not been able to work yet is that when using the '-ps averages' mode, I get this error:

Traceback (most recent call last): File "SparK.py", line 622, in coords.append([get_relative_hight(np.average(averages)), x_pos]) File "SparK.py", line 181, in get_relative_hight return((raw_value hight relative_track_hight_percentage) / max_value) # to not go up to the max TypeError: unsupported operand type(s) for /: 'float' and 'NoneType'

The same command works perfectly well if I omit the '-ps averages' flag (but it overlays separate tracks for each replicate). Can you give me any insight on what might be going wrong for me here?

Cheers!

harbourlab commented 1 year ago

Hey!

Can you send me the complete commands you are using ?

Thanks!

Sent from my iPhone

On Apr 7, 2023, at 3:37 PM, Joe Bowness @.***> wrote:

 CAUTION: This email originated from outside the organization. DO NOT CLICK ON LINKS or OPEN ATTACHMENTS unless you know and trust the sender.

Hello!

Thanks for making this tool! I have recently discovered it, and mostly it is working great for how I want to visualise my tracks.

The only thing I have not been able to work yet is that when using the '-ps averages' mode, I get this error:

Traceback (most recent call last): File "SparK.py", line 622, in coords.append([get_relative_hight(np.average(averages)), x_pos]) File "SparK.py", line 181, in get_relative_hight return((raw_value hight relative_track_hight_percentage) / max_value) # to not go up to the max TypeError: unsupported operand type(s) for /: 'float' and 'NoneType'

The same command works perfectly well if I omit the '-ps averages' flag (but it overlays separate tracks for each replicate). Can you give me any insight on what might be going wrong for me here?

Cheers!

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYRBUFMCX7YWE5SLBWLXABUILANCNFSM6AAAAAAWW3SZGI. You are receiving this because you are subscribed to this thread.Message ID: @.***>

joebowness commented 1 year ago

Hi,

Thanks for your quick reply!

This is an example of a command I am using which works - it generates a plot that looks similar to the one I attach here, with the replicates for each condition overlain separately.

plot Mecp2

python ~/Resources/Software/SparK/SparK.py \ -pr chrX:74082000-74088000 \ -cf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.sort.chrX.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome1.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ -tf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome2.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ -cg 1 2 3 3 4 4 5 5 6 6 -tg 2 3 3 4 4 5 5 6 6 \ -gl unsplit_ES ES d1 d3 d6 d17 \ -l g1 g2 -f 191b80 56fcf1 \ -gtf ~/Resources/gencode.vM25.annotation.chrX.gtf -dg Mecp2 \ -o plots/A11B2_ATAC_Mecp2

However, when I include the flag "-ps averages” into the command, it produces the error I described.

In order to save time and memory, I restrict my bedgraph files to only the region of the genome I am interested in (ie. a ~100Mb region of Chromosome X). Could this potentially produce the issue with the averages function? (Maybe it is trying to divide empty regions?)

Best Wishes, Joe

A11B2_ATAC_Mecp2.pdf

harbourlab commented 1 year ago

Maybe.. could you try using the full bedgraphs real quick?

Sent from my iPhone

On Apr 8, 2023, at 5:26 AM, Joe Bowness @.***> wrote:



Hi,

Thanks for your quick reply!

This is an example of a command I am using which works - it generates a plot that looks similar to the one I attach here, with the replicates for each condition overlain separately.

plot Mecp2

python ~/Resources/Software/SparK/SparK.py \ -pr chrX:74082000-74088000 \ -cf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.sort.chrX.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome1.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ -tf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome2.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ -cg 1 2 3 3 4 4 5 5 6 6 -tg 2 3 3 4 4 5 5 6 6 \ -gl unsplit_ES ES d1 d3 d6 d17 \ -l g1 g2 -f 191b80 56fcf1 \ -gtf ~/Resources/gencode.vM25.annotation.chrX.gtf -dg Mecp2 \ -o plots/A11B2_ATAC_Mecp2

However, when I include the flag "-ps averages” into the command, it produces the error I described.

In order to save time and memory, I restrict my bedgraph files to only the region of the genome I am interested in (ie. a ~100Mb region of Chromosome X). Could this potentially produce the issue with the averages function? (Maybe it is trying to divide empty regions?)

Best Wishes, Joe

On 7 Apr 2023, at 22:59, harbourlab @.***> wrote:

Hey!

Can you send me the complete commands you are using ?

Thanks!

Sent from my iPhone

On Apr 7, 2023, at 3:37 PM, Joe Bowness @.***> wrote:

 CAUTION: This email originated from outside the organization. DO NOT CLICK ON LINKS or OPEN ATTACHMENTS unless you know and trust the sender.

Hello!

Thanks for making this tool! I have recently discovered it, and mostly it is working great for how I want to visualise my tracks.

The only thing I have not been able to work yet is that when using the '-ps averages' mode, I get this error:

Traceback (most recent call last): File "SparK.py", line 622, in coords.append([get_relative_hight(np.average(averages)), x_pos]) File "SparK.py", line 181, in get_relative_hight return((raw_value hight relative_track_hight_percentage) / max_value) # to not go up to the max TypeError: unsupported operand type(s) for /: 'float' and 'NoneType'

The same command works perfectly well if I omit the '-ps averages' flag (but it overlays separate tracks for each replicate). Can you give me any insight on what might be going wrong for me here?

Cheers!

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYRBUFMCX7YWE5SLBWLXABUILANCNFSM6AAAAAAWW3SZGI. You are receiving this because you are subscribed to this thread.Message ID: @.***> — Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22#issuecomment-1500838472, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYXDDZABLVB7DTO7XH3XAEVMHANCNFSM6AAAAAAWW3SZGI. You are receiving this because you commented.Message ID: @.***>

joebowness commented 1 year ago

I just tried with some full genome-wide bedGraphs, and yes, the averaging seems to work fine. So it probably is a problem related to the fact I was trying to use pre-cropped bedGraph files - I guess I will just have to use the full-size ones.

Thanks again for your help.

On 8 Apr 2023, at 13:36, harbourlab @.***> wrote:

Maybe.. could you try using the full bedgraphs real quick?

Sent from my iPhone

On Apr 8, 2023, at 5:26 AM, Joe Bowness @.***> wrote:



Hi,

Thanks for your quick reply!

This is an example of a command I am using which works - it generates a plot that looks similar to the one I attach here, with the replicates for each condition overlain separately.

plot Mecp2

python ~/Resources/Software/SparK/SparK.py \ -pr chrX:74082000-74088000 \ -cf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.sort.chrX.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome1.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ -tf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome2.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ -cg 1 2 3 3 4 4 5 5 6 6 -tg 2 3 3 4 4 5 5 6 6 \ -gl unsplit_ES ES d1 d3 d6 d17 \ -l g1 g2 -f 191b80 56fcf1 \ -gtf ~/Resources/gencode.vM25.annotation.chrX.gtf -dg Mecp2 \ -o plots/A11B2_ATAC_Mecp2

However, when I include the flag "-ps averages” into the command, it produces the error I described.

In order to save time and memory, I restrict my bedgraph files to only the region of the genome I am interested in (ie. a ~100Mb region of Chromosome X). Could this potentially produce the issue with the averages function? (Maybe it is trying to divide empty regions?)

Best Wishes, Joe

On 7 Apr 2023, at 22:59, harbourlab @.***> wrote:

Hey!

Can you send me the complete commands you are using ?

Thanks!

Sent from my iPhone

On Apr 7, 2023, at 3:37 PM, Joe Bowness @.***> wrote:

 CAUTION: This email originated from outside the organization. DO NOT CLICK ON LINKS or OPEN ATTACHMENTS unless you know and trust the sender.

Hello!

Thanks for making this tool! I have recently discovered it, and mostly it is working great for how I want to visualise my tracks.

The only thing I have not been able to work yet is that when using the '-ps averages' mode, I get this error:

Traceback (most recent call last): File "SparK.py", line 622, in coords.append([get_relative_hight(np.average(averages)), x_pos]) File "SparK.py", line 181, in get_relative_hight return((raw_value hight relative_track_hight_percentage) / max_value) # to not go up to the max TypeError: unsupported operand type(s) for /: 'float' and 'NoneType'

The same command works perfectly well if I omit the '-ps averages' flag (but it overlays separate tracks for each replicate). Can you give me any insight on what might be going wrong for me here?

Cheers!

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYRBUFMCX7YWE5SLBWLXABUILANCNFSM6AAAAAAWW3SZGI. You are receiving this because you are subscribed to this thread.Message ID: @.***> — Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22#issuecomment-1500838472, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYXDDZABLVB7DTO7XH3XAEVMHANCNFSM6AAAAAAWW3SZGI. You are receiving this because you commented.Message ID: @.***> — Reply to this email directly, view it on GitHub https://github.com/harbourlab/SparK/issues/22#issuecomment-1500883172, or unsubscribe https://github.com/notifications/unsubscribe-auth/AHZY6M2BMASERGNDOBMONB3XAFLTRANCNFSM6AAAAAAWW3SZGI. You are receiving this because you authored the thread.

harbourlab commented 1 year ago

Great! Glad it works now! Cheers

Sent from my iPhone

On Apr 10, 2023, at 6:12 AM, Joe Bowness @.***> wrote:



I just tried with some full genome-wide bedGraphs, and yes, the averaging seems to work fine. So it probably is a problem related to the fact I was trying to use pre-cropped bedGraph files - I guess I will just have to use the full-size ones.

Thanks again for your help.

On 8 Apr 2023, at 13:36, harbourlab @.***> wrote:

Maybe.. could you try using the full bedgraphs real quick?

Sent from my iPhone

On Apr 8, 2023, at 5:26 AM, Joe Bowness @.***> wrote:



Hi,

Thanks for your quick reply!

This is an example of a command I am using which works - it generates a plot that looks similar to the one I attach here, with the replicates for each condition overlain separately.

plot Mecp2

python ~/Resources/Software/SparK/SparK.py \ -pr chrX:74082000-74088000 \ -cf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.sort.chrX.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome1.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome1.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome1.chrX1.sort.bw.bedGraph.gz \ -tf ~/SparK_tracks/bedGraph/ATAC_A11B2/17_A11B2_ES_ATAC.genome2.sort.chrX1.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_1d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_3d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_6d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep1.genome2.chrX1.sort.bw.bedGraph.gz ~/SparK_tracks/bedGraph/ATAC_A11B2/NPC_17d_Rep2.genome2.chrX1.sort.bw.bedGraph.gz \ -cg 1 2 3 3 4 4 5 5 6 6 -tg 2 3 3 4 4 5 5 6 6 \ -gl unsplit_ES ES d1 d3 d6 d17 \ -l g1 g2 -f 191b80 56fcf1 \ -gtf ~/Resources/gencode.vM25.annotation.chrX.gtf -dg Mecp2 \ -o plots/A11B2_ATAC_Mecp2

However, when I include the flag "-ps averages” into the command, it produces the error I described.

In order to save time and memory, I restrict my bedgraph files to only the region of the genome I am interested in (ie. a ~100Mb region of Chromosome X). Could this potentially produce the issue with the averages function? (Maybe it is trying to divide empty regions?)

Best Wishes, Joe

On 7 Apr 2023, at 22:59, harbourlab @.***> wrote:

Hey!

Can you send me the complete commands you are using ?

Thanks!

Sent from my iPhone

On Apr 7, 2023, at 3:37 PM, Joe Bowness @.***> wrote:

 CAUTION: This email originated from outside the organization. DO NOT CLICK ON LINKS or OPEN ATTACHMENTS unless you know and trust the sender.

Hello!

Thanks for making this tool! I have recently discovered it, and mostly it is working great for how I want to visualise my tracks.

The only thing I have not been able to work yet is that when using the '-ps averages' mode, I get this error:

Traceback (most recent call last): File "SparK.py", line 622, in coords.append([get_relative_hight(np.average(averages)), x_pos]) File "SparK.py", line 181, in get_relative_hight return((raw_value hight relative_track_hight_percentage) / max_value) # to not go up to the max TypeError: unsupported operand type(s) for /: 'float' and 'NoneType'

The same command works perfectly well if I omit the '-ps averages' flag (but it overlays separate tracks for each replicate). Can you give me any insight on what might be going wrong for me here?

Cheers!

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYRBUFMCX7YWE5SLBWLXABUILANCNFSM6AAAAAAWW3SZGI. You are receiving this because you are subscribed to this thread.Message ID: @.***> — Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22#issuecomment-1500838472, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYXDDZABLVB7DTO7XH3XAEVMHANCNFSM6AAAAAAWW3SZGI. You are receiving this because you commented.Message ID: @.***> — Reply to this email directly, view it on GitHub https://github.com/harbourlab/SparK/issues/22#issuecomment-1500883172, or unsubscribe https://github.com/notifications/unsubscribe-auth/AHZY6M2BMASERGNDOBMONB3XAFLTRANCNFSM6AAAAAAWW3SZGI. You are receiving this because you authored the thread.

— Reply to this email directly, view it on GitHubhttps://github.com/harbourlab/SparK/issues/22#issuecomment-1501642893, or unsubscribehttps://github.com/notifications/unsubscribe-auth/AHANDYULGAKXFIOWUUL6KULXAPMIBANCNFSM6AAAAAAWW3SZGI. You are receiving this because you commented.Message ID: @.***>