hartleys / QoRTs

Quality of RNA-Seq Toolset
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Definition of good and bad overlapped read pairs #91

Open SJJHK opened 9 months ago

SJJHK commented 9 months ago

Thanks for the package!

We have an issue defining "good" and "bad" in the below lines in LARGE FONT and in BOLD extracted from the summary.txt. file of QoRTs' QC data.

OM_noOverlap_staggered 0 Number of read-pairs with no overlap, mis-staggered such that the fwd read occurs second. OM_noOverlap_normal 1069491 Number of read-pairs with no overlap, arranged normally. OM_overlap 22736461 Number of read-pairs with good overlap. OM_BAD_OVERLAP 1929 Number of read-pairs with a bad overlap. OM_overlap_Match 20928805 Number of read-pairs with a good overlap, and that match perfectly. OM_overlap_mismatch 1807656 Number of read-pairs with a good overlap, but that contain one or more base mismatches.

I have looked for more detail in relation to the summary.txt file - is there somewhere I can find more detail for the summary text outputs?

Thanks in advance, Sam