Closed msubirana closed 1 year ago
Hi,
Do you have the files /tmp/ratioSegmentation.R14431321662434737205.error
and /tmp/ratioSegmentation.R14431321662434737205.error
? They should contain the actual errors.
The files do no exists...
And the /tmp/script17671355960897683989.R also does not exist
I see. Is it possible that the /tmp directory is not writable?
yes, I'm running in the cluster and I have limited rights. Is it possible to redefine the tmp path?
The source code uses java standard method to create a temp file. We do not explicitly control how the tmp path is managed, as it is different between operating systems, and therefore beyond our scope.
Would you mind pasting the command line argument for cobalt here, and also the bam file header? You can get the bam header with the command:
samtools view -H <bam file>
amber=/gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/refs/hmftools/amber-3.9.jar
cobalt=/gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/refs/hmftools/cobalt-1.14.jar
tumor_name=NET25_TI
tumor_bam=/gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/INS/all_ok/NET25_TI.bam
ctrl_name=NET25_BL
ctrl_bam=/gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/INS/all_ok/NET25_BL.bam
output_dir=/gpfs42/robbyfs/scratch/lab_lpasquali/shared_data/projects-data/insulinomas-genetics/output/hmftools
GermlineHetPon=/gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/refs/HMFtools-Resources_dna_pipeline_v5_32_38_hmf_dna_pipeline_resources.38_v5.32/copy_number/GermlineHetPon.38.vcf.gz
GC_profile=/gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/refs/HMFtools-Resources_dna_pipeline_v5_32_38_hmf_dna_pipeline_resources.38_v5.32/copy_number/GC_profile.1000bp.38.cnp
output_amber=${output_dir}/amber
output_cobalt=${output_dir}/cobalt
threads=16
mkdir -p $output_amber
mkdir -p $output_cobalt
# cobalt
java -jar -Xmx8G $cobalt -reference $ctrl_name -reference_bam $ctrl_bam -tumor $tumor_name -tumor_bam $tumor_bam -output_dir $output_cobalt -threads $threads -gc_profile $GC_profile
@HD VN:1.6 SO:coordinate
@SQ SN:chr1 LN:248956422
@SQ SN:chr2 LN:242193529
@SQ SN:chr3 LN:198295559
@SQ SN:chr4 LN:190214555
@SQ SN:chr5 LN:181538259
@SQ SN:chr6 LN:170805979
@SQ SN:chr7 LN:159345973
@SQ SN:chr8 LN:145138636
@SQ SN:chr9 LN:138394717
@SQ SN:chr10 LN:133797422
@SQ SN:chr11 LN:135086622
@SQ SN:chr12 LN:133275309
@SQ SN:chr13 LN:114364328
@SQ SN:chr14 LN:107043718
@SQ SN:chr15 LN:101991189
@SQ SN:chr16 LN:90338345
@SQ SN:chr17 LN:83257441
@SQ SN:chr18 LN:80373285
@SQ SN:chr19 LN:58617616
@SQ SN:chr20 LN:64444167
@SQ SN:chr21 LN:46709983
@SQ SN:chr22 LN:50818468
@SQ SN:chrX LN:156040895
@SQ SN:chrY LN:57227415
@SQ SN:chrM LN:16569
@SQ SN:chr1_KI270706v1_random LN:175055
@SQ SN:chr1_KI270707v1_random LN:32032
@SQ SN:chr1_KI270708v1_random LN:127682
@SQ SN:chr1_KI270709v1_random LN:66860
@SQ SN:chr1_KI270710v1_random LN:40176
@SQ SN:chr1_KI270711v1_random LN:42210
@SQ SN:chr1_KI270712v1_random LN:176043
@SQ SN:chr1_KI270713v1_random LN:40745
@SQ SN:chr1_KI270714v1_random LN:41717
@SQ SN:chr2_KI270715v1_random LN:161471
@SQ SN:chr2_KI270716v1_random LN:153799
@SQ SN:chr3_GL000221v1_random LN:155397
@SQ SN:chr4_GL000008v2_random LN:209709
@SQ SN:chr5_GL000208v1_random LN:92689
@SQ SN:chr9_KI270717v1_random LN:40062
@SQ SN:chr9_KI270718v1_random LN:38054
@SQ SN:chr9_KI270719v1_random LN:176845
@SQ SN:chr9_KI270720v1_random LN:39050
@SQ SN:chr11_KI270721v1_random LN:100316
@SQ SN:chr14_GL000009v2_random LN:201709
@SQ SN:chr14_GL000225v1_random LN:211173
@SQ SN:chr14_KI270722v1_random LN:194050
@SQ SN:chr14_GL000194v1_random LN:191469
@SQ SN:chr14_KI270723v1_random LN:38115
@SQ SN:chr14_KI270724v1_random LN:39555
@SQ SN:chr14_KI270725v1_random LN:172810
@SQ SN:chr14_KI270726v1_random LN:43739
@SQ SN:chr15_KI270727v1_random LN:448248
@SQ SN:chr16_KI270728v1_random LN:1872759
@SQ SN:chr17_GL000205v2_random LN:185591
@SQ SN:chr17_KI270729v1_random LN:280839
@SQ SN:chr17_KI270730v1_random LN:112551
@SQ SN:chr22_KI270731v1_random LN:150754
@SQ SN:chr22_KI270732v1_random LN:41543
@SQ SN:chr22_KI270733v1_random LN:179772
@SQ SN:chr22_KI270734v1_random LN:165050
@SQ SN:chr22_KI270735v1_random LN:42811
@SQ SN:chr22_KI270736v1_random LN:181920
@SQ SN:chr22_KI270737v1_random LN:103838
@SQ SN:chr22_KI270738v1_random LN:99375
@SQ SN:chr22_KI270739v1_random LN:73985
@SQ SN:chrY_KI270740v1_random LN:37240
@SQ SN:chrUn_KI270302v1 LN:2274
@SQ SN:chrUn_KI270304v1 LN:2165
@SQ SN:chrUn_KI270303v1 LN:1942
@SQ SN:chrUn_KI270305v1 LN:1472
@SQ SN:chrUn_KI270322v1 LN:21476
@SQ SN:chrUn_KI270320v1 LN:4416
@SQ SN:chrUn_KI270310v1 LN:1201
@SQ SN:chrUn_KI270316v1 LN:1444
@SQ SN:chrUn_KI270315v1 LN:2276
@SQ SN:chrUn_KI270312v1 LN:998
@SQ SN:chrUn_KI270311v1 LN:12399
@SQ SN:chrUn_KI270317v1 LN:37690
@SQ SN:chrUn_KI270412v1 LN:1179
@SQ SN:chrUn_KI270411v1 LN:2646
@SQ SN:chrUn_KI270414v1 LN:2489
@SQ SN:chrUn_KI270419v1 LN:1029
@SQ SN:chrUn_KI270418v1 LN:2145
@SQ SN:chrUn_KI270420v1 LN:2321
@SQ SN:chrUn_KI270424v1 LN:2140
@SQ SN:chrUn_KI270417v1 LN:2043
@SQ SN:chrUn_KI270422v1 LN:1445
@SQ SN:chrUn_KI270423v1 LN:981
@SQ SN:chrUn_KI270425v1 LN:1884
@SQ SN:chrUn_KI270429v1 LN:1361
@SQ SN:chrUn_KI270442v1 LN:392061
@SQ SN:chrUn_KI270466v1 LN:1233
@SQ SN:chrUn_KI270465v1 LN:1774
@SQ SN:chrUn_KI270467v1 LN:3920
@SQ SN:chrUn_KI270435v1 LN:92983
@SQ SN:chrUn_KI270438v1 LN:112505
@SQ SN:chrUn_KI270468v1 LN:4055
@SQ SN:chrUn_KI270510v1 LN:2415
@SQ SN:chrUn_KI270509v1 LN:2318
@SQ SN:chrUn_KI270518v1 LN:2186
@SQ SN:chrUn_KI270508v1 LN:1951
@SQ SN:chrUn_KI270516v1 LN:1300
@SQ SN:chrUn_KI270512v1 LN:22689
@SQ SN:chrUn_KI270519v1 LN:138126
@SQ SN:chrUn_KI270522v1 LN:5674
@SQ SN:chrUn_KI270511v1 LN:8127
@SQ SN:chrUn_KI270515v1 LN:6361
@SQ SN:chrUn_KI270507v1 LN:5353
@SQ SN:chrUn_KI270517v1 LN:3253
@SQ SN:chrUn_KI270529v1 LN:1899
@SQ SN:chrUn_KI270528v1 LN:2983
@SQ SN:chrUn_KI270530v1 LN:2168
@SQ SN:chrUn_KI270539v1 LN:993
@SQ SN:chrUn_KI270538v1 LN:91309
@SQ SN:chrUn_KI270544v1 LN:1202
@SQ SN:chrUn_KI270548v1 LN:1599
@SQ SN:chrUn_KI270583v1 LN:1400
@SQ SN:chrUn_KI270587v1 LN:2969
@SQ SN:chrUn_KI270580v1 LN:1553
@SQ SN:chrUn_KI270581v1 LN:7046
@SQ SN:chrUn_KI270579v1 LN:31033
@SQ SN:chrUn_KI270589v1 LN:44474
@SQ SN:chrUn_KI270590v1 LN:4685
@SQ SN:chrUn_KI270584v1 LN:4513
@SQ SN:chrUn_KI270582v1 LN:6504
@SQ SN:chrUn_KI270588v1 LN:6158
@SQ SN:chrUn_KI270593v1 LN:3041
@SQ SN:chrUn_KI270591v1 LN:5796
@SQ SN:chrUn_KI270330v1 LN:1652
@SQ SN:chrUn_KI270329v1 LN:1040
@SQ SN:chrUn_KI270334v1 LN:1368
@SQ SN:chrUn_KI270333v1 LN:2699
@SQ SN:chrUn_KI270335v1 LN:1048
@SQ SN:chrUn_KI270338v1 LN:1428
@SQ SN:chrUn_KI270340v1 LN:1428
@SQ SN:chrUn_KI270336v1 LN:1026
@SQ SN:chrUn_KI270337v1 LN:1121
@SQ SN:chrUn_KI270363v1 LN:1803
@SQ SN:chrUn_KI270364v1 LN:2855
@SQ SN:chrUn_KI270362v1 LN:3530
@SQ SN:chrUn_KI270366v1 LN:8320
@SQ SN:chrUn_KI270378v1 LN:1048
@SQ SN:chrUn_KI270379v1 LN:1045
@SQ SN:chrUn_KI270389v1 LN:1298
@SQ SN:chrUn_KI270390v1 LN:2387
@SQ SN:chrUn_KI270387v1 LN:1537
@SQ SN:chrUn_KI270395v1 LN:1143
@SQ SN:chrUn_KI270396v1 LN:1880
@SQ SN:chrUn_KI270388v1 LN:1216
@SQ SN:chrUn_KI270394v1 LN:970
@SQ SN:chrUn_KI270386v1 LN:1788
@SQ SN:chrUn_KI270391v1 LN:1484
@SQ SN:chrUn_KI270383v1 LN:1750
@SQ SN:chrUn_KI270393v1 LN:1308
@SQ SN:chrUn_KI270384v1 LN:1658
@SQ SN:chrUn_KI270392v1 LN:971
@SQ SN:chrUn_KI270381v1 LN:1930
@SQ SN:chrUn_KI270385v1 LN:990
@SQ SN:chrUn_KI270382v1 LN:4215
@SQ SN:chrUn_KI270376v1 LN:1136
@SQ SN:chrUn_KI270374v1 LN:2656
@SQ SN:chrUn_KI270372v1 LN:1650
@SQ SN:chrUn_KI270373v1 LN:1451
@SQ SN:chrUn_KI270375v1 LN:2378
@SQ SN:chrUn_KI270371v1 LN:2805
@SQ SN:chrUn_KI270448v1 LN:7992
@SQ SN:chrUn_KI270521v1 LN:7642
@SQ SN:chrUn_GL000195v1 LN:182896
@SQ SN:chrUn_GL000219v1 LN:179198
@SQ SN:chrUn_GL000220v1 LN:161802
@SQ SN:chrUn_GL000224v1 LN:179693
@SQ SN:chrUn_KI270741v1 LN:157432
@SQ SN:chrUn_GL000226v1 LN:15008
@SQ SN:chrUn_GL000213v1 LN:164239
@SQ SN:chrUn_KI270743v1 LN:210658
@SQ SN:chrUn_KI270744v1 LN:168472
@SQ SN:chrUn_KI270745v1 LN:41891
@SQ SN:chrUn_KI270746v1 LN:66486
@SQ SN:chrUn_KI270747v1 LN:198735
@SQ SN:chrUn_KI270748v1 LN:93321
@SQ SN:chrUn_KI270749v1 LN:158759
@SQ SN:chrUn_KI270750v1 LN:148850
@SQ SN:chrUn_KI270751v1 LN:150742
@SQ SN:chrUn_KI270752v1 LN:27745
@SQ SN:chrUn_KI270753v1 LN:62944
@SQ SN:chrUn_KI270754v1 LN:40191
@SQ SN:chrUn_KI270755v1 LN:36723
@SQ SN:chrUn_KI270756v1 LN:79590
@SQ SN:chrUn_KI270757v1 LN:71251
@SQ SN:chrUn_GL000214v1 LN:137718
@SQ SN:chrUn_KI270742v1 LN:186739
@SQ SN:chrUn_GL000216v2 LN:176608
@SQ SN:chrUn_GL000218v1 LN:161147
@SQ SN:chrEBV LN:171823
@RG ID:NET25_BL PL:ILLUMINA PU:1 SM:NET25_BL
@PG ID:bwa PN:bwa VN:0.7.17-r1194-dirty CL:bwa mem -M -t 48 -R @RG\tID:NET25_BL\tPL:ILLUMINA\tPU:1\tSM:NET25_BL /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/ref/GCA_000001405.15_GRCh38_no_alt_analysis_set.fa /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/toDo/NET25_BL_R1.fq /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/toDo/NET25_BL_R2.fq
@PG ID:SAMBLASTER VN:0.1.24 CL:samblaster -i stdin -o stdout -M
@PG ID:samtools PN:samtools PP:SAMBLASTER VN:1.12 CL:samtools view -H /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/INS/all_ok/NET25_BL.bam
@HD VN:1.6 SO:coordinate
@SQ SN:chr1 LN:248956422
@SQ SN:chr2 LN:242193529
@SQ SN:chr3 LN:198295559
@SQ SN:chr4 LN:190214555
@SQ SN:chr5 LN:181538259
@SQ SN:chr6 LN:170805979
@SQ SN:chr7 LN:159345973
@SQ SN:chr8 LN:145138636
@SQ SN:chr9 LN:138394717
@SQ SN:chr10 LN:133797422
@SQ SN:chr11 LN:135086622
@SQ SN:chr12 LN:133275309
@SQ SN:chr13 LN:114364328
@SQ SN:chr14 LN:107043718
@SQ SN:chr15 LN:101991189
@SQ SN:chr16 LN:90338345
@SQ SN:chr17 LN:83257441
@SQ SN:chr18 LN:80373285
@SQ SN:chr19 LN:58617616
@SQ SN:chr20 LN:64444167
@SQ SN:chr21 LN:46709983
@SQ SN:chr22 LN:50818468
@SQ SN:chrX LN:156040895
@SQ SN:chrY LN:57227415
@SQ SN:chrM LN:16569
@SQ SN:chr1_KI270706v1_random LN:175055
@SQ SN:chr1_KI270707v1_random LN:32032
@SQ SN:chr1_KI270708v1_random LN:127682
@SQ SN:chr1_KI270709v1_random LN:66860
@SQ SN:chr1_KI270710v1_random LN:40176
@SQ SN:chr1_KI270711v1_random LN:42210
@SQ SN:chr1_KI270712v1_random LN:176043
@SQ SN:chr1_KI270713v1_random LN:40745
@SQ SN:chr1_KI270714v1_random LN:41717
@SQ SN:chr2_KI270715v1_random LN:161471
@SQ SN:chr2_KI270716v1_random LN:153799
@SQ SN:chr3_GL000221v1_random LN:155397
@SQ SN:chr4_GL000008v2_random LN:209709
@SQ SN:chr5_GL000208v1_random LN:92689
@SQ SN:chr9_KI270717v1_random LN:40062
@SQ SN:chr9_KI270718v1_random LN:38054
@SQ SN:chr9_KI270719v1_random LN:176845
@SQ SN:chr9_KI270720v1_random LN:39050
@SQ SN:chr11_KI270721v1_random LN:100316
@SQ SN:chr14_GL000009v2_random LN:201709
@SQ SN:chr14_GL000225v1_random LN:211173
@SQ SN:chr14_KI270722v1_random LN:194050
@SQ SN:chr14_GL000194v1_random LN:191469
@SQ SN:chr14_KI270723v1_random LN:38115
@SQ SN:chr14_KI270724v1_random LN:39555
@SQ SN:chr14_KI270725v1_random LN:172810
@SQ SN:chr14_KI270726v1_random LN:43739
@SQ SN:chr15_KI270727v1_random LN:448248
@SQ SN:chr16_KI270728v1_random LN:1872759
@SQ SN:chr17_GL000205v2_random LN:185591
@SQ SN:chr17_KI270729v1_random LN:280839
@SQ SN:chr17_KI270730v1_random LN:112551
@SQ SN:chr22_KI270731v1_random LN:150754
@SQ SN:chr22_KI270732v1_random LN:41543
@SQ SN:chr22_KI270733v1_random LN:179772
@SQ SN:chr22_KI270734v1_random LN:165050
@SQ SN:chr22_KI270735v1_random LN:42811
@SQ SN:chr22_KI270736v1_random LN:181920
@SQ SN:chr22_KI270737v1_random LN:103838
@SQ SN:chr22_KI270738v1_random LN:99375
@SQ SN:chr22_KI270739v1_random LN:73985
@SQ SN:chrY_KI270740v1_random LN:37240
@SQ SN:chrUn_KI270302v1 LN:2274
@SQ SN:chrUn_KI270304v1 LN:2165
@SQ SN:chrUn_KI270303v1 LN:1942
@SQ SN:chrUn_KI270305v1 LN:1472
@SQ SN:chrUn_KI270322v1 LN:21476
@SQ SN:chrUn_KI270320v1 LN:4416
@SQ SN:chrUn_KI270310v1 LN:1201
@SQ SN:chrUn_KI270316v1 LN:1444
@SQ SN:chrUn_KI270315v1 LN:2276
@SQ SN:chrUn_KI270312v1 LN:998
@SQ SN:chrUn_KI270311v1 LN:12399
@SQ SN:chrUn_KI270317v1 LN:37690
@SQ SN:chrUn_KI270412v1 LN:1179
@SQ SN:chrUn_KI270411v1 LN:2646
@SQ SN:chrUn_KI270414v1 LN:2489
@SQ SN:chrUn_KI270419v1 LN:1029
@SQ SN:chrUn_KI270418v1 LN:2145
@SQ SN:chrUn_KI270420v1 LN:2321
@SQ SN:chrUn_KI270424v1 LN:2140
@SQ SN:chrUn_KI270417v1 LN:2043
@SQ SN:chrUn_KI270422v1 LN:1445
@SQ SN:chrUn_KI270423v1 LN:981
@SQ SN:chrUn_KI270425v1 LN:1884
@SQ SN:chrUn_KI270429v1 LN:1361
@SQ SN:chrUn_KI270442v1 LN:392061
@SQ SN:chrUn_KI270466v1 LN:1233
@SQ SN:chrUn_KI270465v1 LN:1774
@SQ SN:chrUn_KI270467v1 LN:3920
@SQ SN:chrUn_KI270435v1 LN:92983
@SQ SN:chrUn_KI270438v1 LN:112505
@SQ SN:chrUn_KI270468v1 LN:4055
@SQ SN:chrUn_KI270510v1 LN:2415
@SQ SN:chrUn_KI270509v1 LN:2318
@SQ SN:chrUn_KI270518v1 LN:2186
@SQ SN:chrUn_KI270508v1 LN:1951
@SQ SN:chrUn_KI270516v1 LN:1300
@SQ SN:chrUn_KI270512v1 LN:22689
@SQ SN:chrUn_KI270519v1 LN:138126
@SQ SN:chrUn_KI270522v1 LN:5674
@SQ SN:chrUn_KI270511v1 LN:8127
@SQ SN:chrUn_KI270515v1 LN:6361
@SQ SN:chrUn_KI270507v1 LN:5353
@SQ SN:chrUn_KI270517v1 LN:3253
@SQ SN:chrUn_KI270529v1 LN:1899
@SQ SN:chrUn_KI270528v1 LN:2983
@SQ SN:chrUn_KI270530v1 LN:2168
@SQ SN:chrUn_KI270539v1 LN:993
@SQ SN:chrUn_KI270538v1 LN:91309
@SQ SN:chrUn_KI270544v1 LN:1202
@SQ SN:chrUn_KI270548v1 LN:1599
@SQ SN:chrUn_KI270583v1 LN:1400
@SQ SN:chrUn_KI270587v1 LN:2969
@SQ SN:chrUn_KI270580v1 LN:1553
@SQ SN:chrUn_KI270581v1 LN:7046
@SQ SN:chrUn_KI270579v1 LN:31033
@SQ SN:chrUn_KI270589v1 LN:44474
@SQ SN:chrUn_KI270590v1 LN:4685
@SQ SN:chrUn_KI270584v1 LN:4513
@SQ SN:chrUn_KI270582v1 LN:6504
@SQ SN:chrUn_KI270588v1 LN:6158
@SQ SN:chrUn_KI270593v1 LN:3041
@SQ SN:chrUn_KI270591v1 LN:5796
@SQ SN:chrUn_KI270330v1 LN:1652
@SQ SN:chrUn_KI270329v1 LN:1040
@SQ SN:chrUn_KI270334v1 LN:1368
@SQ SN:chrUn_KI270333v1 LN:2699
@SQ SN:chrUn_KI270335v1 LN:1048
@SQ SN:chrUn_KI270338v1 LN:1428
@SQ SN:chrUn_KI270340v1 LN:1428
@SQ SN:chrUn_KI270336v1 LN:1026
@SQ SN:chrUn_KI270337v1 LN:1121
@SQ SN:chrUn_KI270363v1 LN:1803
@SQ SN:chrUn_KI270364v1 LN:2855
@SQ SN:chrUn_KI270362v1 LN:3530
@SQ SN:chrUn_KI270366v1 LN:8320
@SQ SN:chrUn_KI270378v1 LN:1048
@SQ SN:chrUn_KI270379v1 LN:1045
@SQ SN:chrUn_KI270389v1 LN:1298
@SQ SN:chrUn_KI270390v1 LN:2387
@SQ SN:chrUn_KI270387v1 LN:1537
@SQ SN:chrUn_KI270395v1 LN:1143
@SQ SN:chrUn_KI270396v1 LN:1880
@SQ SN:chrUn_KI270388v1 LN:1216
@SQ SN:chrUn_KI270394v1 LN:970
@SQ SN:chrUn_KI270386v1 LN:1788
@SQ SN:chrUn_KI270391v1 LN:1484
@SQ SN:chrUn_KI270383v1 LN:1750
@SQ SN:chrUn_KI270393v1 LN:1308
@SQ SN:chrUn_KI270384v1 LN:1658
@SQ SN:chrUn_KI270392v1 LN:971
@SQ SN:chrUn_KI270381v1 LN:1930
@SQ SN:chrUn_KI270385v1 LN:990
@SQ SN:chrUn_KI270382v1 LN:4215
@SQ SN:chrUn_KI270376v1 LN:1136
@SQ SN:chrUn_KI270374v1 LN:2656
@SQ SN:chrUn_KI270372v1 LN:1650
@SQ SN:chrUn_KI270373v1 LN:1451
@SQ SN:chrUn_KI270375v1 LN:2378
@SQ SN:chrUn_KI270371v1 LN:2805
@SQ SN:chrUn_KI270448v1 LN:7992
@SQ SN:chrUn_KI270521v1 LN:7642
@SQ SN:chrUn_GL000195v1 LN:182896
@SQ SN:chrUn_GL000219v1 LN:179198
@SQ SN:chrUn_GL000220v1 LN:161802
@SQ SN:chrUn_GL000224v1 LN:179693
@SQ SN:chrUn_KI270741v1 LN:157432
@SQ SN:chrUn_GL000226v1 LN:15008
@SQ SN:chrUn_GL000213v1 LN:164239
@SQ SN:chrUn_KI270743v1 LN:210658
@SQ SN:chrUn_KI270744v1 LN:168472
@SQ SN:chrUn_KI270745v1 LN:41891
@SQ SN:chrUn_KI270746v1 LN:66486
@SQ SN:chrUn_KI270747v1 LN:198735
@SQ SN:chrUn_KI270748v1 LN:93321
@SQ SN:chrUn_KI270749v1 LN:158759
@SQ SN:chrUn_KI270750v1 LN:148850
@SQ SN:chrUn_KI270751v1 LN:150742
@SQ SN:chrUn_KI270752v1 LN:27745
@SQ SN:chrUn_KI270753v1 LN:62944
@SQ SN:chrUn_KI270754v1 LN:40191
@SQ SN:chrUn_KI270755v1 LN:36723
@SQ SN:chrUn_KI270756v1 LN:79590
@SQ SN:chrUn_KI270757v1 LN:71251
@SQ SN:chrUn_GL000214v1 LN:137718
@SQ SN:chrUn_KI270742v1 LN:186739
@SQ SN:chrUn_GL000216v2 LN:176608
@SQ SN:chrUn_GL000218v1 LN:161147
@SQ SN:chrEBV LN:171823
@RG ID:NET25_TI PL:ILLUMINA PU:1 SM:NET25_TI
@PG ID:bwa PN:bwa VN:0.7.17-r1194-dirty CL:bwa mem -M -t 48 -R @RG\tID:NET25_TI\tPL:ILLUMINA\tPU:1\tSM:NET25_TI /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/ref/GCA_000001405.15_GRCh38_no_alt_analysis_set.fa /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/toDo/NET25_TI_R1.fq /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/toDo/NET25_TI_R2.fq
@PG ID:SAMBLASTER VN:0.1.24 CL:samblaster -i stdin -o stdout -M
@PG ID:samtools PN:samtools PP:SAMBLASTER VN:1.12 CL:samtools view -H /gpfs42/robbyfs/scratch/lab_lpasquali/msubirana/marc/insulinomas/data/WGS/BAM/INS/all_ok/NET25_TI.bam
Thanks. This all seem fine to me. Would you be able try running it on a machine with writable /tmp directory and see if it works? Just want to make sure the /tmp is the issue here.
Is it possible to run the R script separately to have all the files that purple requires from cobalt and amber?
You can extract the script out from the repo and run them manually. Though I suspect making /tmp writable would be a much simpler option?
Are you able to try running the R script manually and see if it works?
Rscript ratioSegmentation.R /gpfs42/robbyfs/scratch/lab_lpasquali/shared_data/projects-data/insulinomas-genetics/output/gpl/NET25_TI/cobalt/NET25_TI.cobalt.ratio.tsv.gz tumorGCRatio /gpfs42/robbyfs/scratch/lab_lpasquali/shared_data/projects-data/insulinomas-genetics/output/gpl/NET25_TI/cobalt/NET25_TI.cobalt.ratio.pcf 100
I just found out that there is indeed a way to set the tmp directory for java process, when executing the java command, add the following -Djava.io.tmpdir=<new temp dir>
, i.e.
java -Djava.io.tmpdir=/path/to/tmpdir -jar cobalt.jar ...
sorry for the late replay, I tested the ratioSegmentation.R from the repo and the copynumber dependence was not correctly installed, after check all the dependencies it works! thanks for all the feedback!
I'm running the pipeline using:
./pipeline/scripts/run_pipeline ./pipeline/scripts data HMFtools-Resources_dna_pipeline_v5_32_38_hmf_dna_pipeline_resources.38_v5.32 HMFtools-Resources_dna_pipeline_v5_32_hmf_dna_pipeline_tools_v5.32 NET25_TI,NET25_BL V38 WGS 10 16
But during the R runs, it breaks. I have installed all the R dependencies, but it seems that the /tmp/script17671355960897683989.R file is not present. Then, Purple does not have the .cobalt.ratio.pcf files, and it breaks. Do you have any suggestions?