hartwigmedical / hmftools

Various algorithms for analysing genomics data
GNU General Public License v3.0
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SAGE error #443

Closed alhafidzhamdan closed 11 months ago

alhafidzhamdan commented 11 months ago

Hi all,

I'm using SAGE v3.3 and resources v5.33

java $JVM_OPTS $JVM_TMP_DIR -cp $SAGE_JAR com.hartwig.hmftools.sage.SageApplication \
      -threads 16 \
      -reference ${PATIENT_ID}N -reference_bam $ALIGNED_BAM_FILE_NORMAL \
      -tumor ${PATIENT_ID}${TYPE} -tumor_bam $ALIGNED_BAM_FILE_TUMOR \
      -ref_genome_version 38 \
      -ref_genome $REFERENCE \
      -hotspots $SOMATIC_HOTSPOTS_V533 \
      -panel_bed $SOMATIC_ACTIONABLE_V533 \
      -high_confidence_bed $HIGH_CONF_BED_V533 \
      -ensembl_data_dir $HMF_ENSEMBLE_V533 \
      -hotspot_min_tumor_qual 40 \
      -panel_min_tumor_qual 60 \
      -high_confidence_min_tumor_qual 100 \
      -low_confidence_min_tumor_qual 150 \
      -include_mt \
      -out $SAGE_VCF

I got this error:

12:19:01.605 [INFO ] Sage version: 3.3
Exception in thread "main" java.lang.NullPointerException
        at com.hartwig.hmftools.common.hla.HlaCommon.populateGeneData(HlaCommon.java:29)
        at com.hartwig.hmftools.sage.ReferenceData.<init>(ReferenceData.java:63)
        at com.hartwig.hmftools.sage.SageApplication.<init>(SageApplication.java:50)
        at com.hartwig.hmftools.sage.SageApplication.main(SageApplication.java:146)

When i ran Sage v3.3 with resources version 5.28, it ran til completion.

Could you please help me troubleshoot?