harvardinformatics / snpArcher

Snakemake workflow for highly parallel variant calling designed for ease-of-use in non-model organisms.
MIT License
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Error with running the command #200

Closed AmitSharma267 closed 6 days ago

AmitSharma267 commented 1 week ago

Hi there,

when we are trying to run the command we are encountering this error.

image

the sample sheet is set up as:

BioSample,refGenome,refPath,fq1,fq2
SS3_S143,sclscl_ref,/home/sharma267/project1/GCF_000146945.2_ASM14694v2_genomic.fna,/home/sharma267/project1/SS3_S143_L008_R1_001.fastq.gz,/home/sharma267/project1/SS3_S143_L008_R2_001.fastq.gz

and config.yaml looks like this:

image

Also tried putting the reference genome and its path to the config.yaml file but it gives the same error.

We are unable to figure out root of the problem. Could you please help me here? Please let me know if more information is needed. Thanks in advance. Amit

erikenbody commented 1 week ago

Looks like you are missing the “run” column form the sample sheet

On Fri, Jun 7, 2024 at 12:57 PM AmitSharma267 @.***> wrote:

Hi there,

when we are trying to run the command we are encountering this error. image.png (view on web) https://github.com/harvardinformatics/snpArcher/assets/158214436/ce8d2ac2-38a4-452b-986f-9068d940d5fe

the sample sheet is set up as:

BioSample,refGenome,refPath,fq1,fq2 SS3_S143,sclscl_ref,/home/sharma267/project1/GCF_000146945.2_ASM14694v2_genomic.fna,/home/sharma267/project1/SS3_S143_L008_R1_001.fastq.gz,/home/sharma267/project1/SS3_S143_L008_R2_001.fastq.gz

and config.yaml looks like this: image.png (view on web) https://github.com/harvardinformatics/snpArcher/assets/158214436/bb168292-2e79-4ff5-bc7e-5a6ff90d2448

Also tried putting the reference genome and its path to the config.yaml file but it gives the same error.

We are unable to figure out root of the problem. Could you please help me here? Please let me know if more information is needed. Thanks in advance. Amit

— Reply to this email directly, view it on GitHub https://github.com/harvardinformatics/snpArcher/issues/200, or unsubscribe https://github.com/notifications/unsubscribe-auth/ADBA3NYW3UVEJCXFYWITO6LZGIGDNAVCNFSM6AAAAABI7HG3EKVHI2DSMVQWIX3LMV43ASLTON2WKOZSGM2DCMBXHA2TMNI . You are receiving this because you are subscribed to this thread.Message ID: @.***>

AmitSharma267 commented 6 days ago

That error is gone. Thank you