search

hasindu2008 / f5c

Ultra-fast methylation calling and event alignment tool for nanopore sequencing data (supports CUDA acceleration)
https://hasindu2008.github.io/f5c/docs/overview
MIT License
144 stars 26 forks source link

[get_rank::WARNING] A None ACGT base found : U #106

Closed JeremyQuo closed 2 years ago

JeremyQuo commented 2 years ago

When I use f5c call-methylation, it return this warning [get_rank::WARNING] A None ACGT base found : U.

Will it influence the results? Can --rna option only applied on the evenalign?

JeremyQuo commented 2 years ago

My data is rna

hasindu2008 commented 2 years ago

Unfortunately, f5c/nanopolish does not have a methylation model available for RNA. So methylation calling only works for DNA.