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hbctraining / scRNA-seq_online

https://hbctraining.github.io/scRNA-seq_online/.
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Quality Control visualizations #110

Closed chenqing1991 closed 1 month ago

chenqing1991 commented 10 months ago

Hi，I have just learned the scRNA-seq_online course，I have a question

when you visualiza the UMI counts (transcripts) per cell by code as bellow:

Visualize the number UMIs/transcripts per cell

metadata %>% ggplot(aes(color=sample, x=nUMI, fill= sample)) + geom_density(alpha = 0.2) + scale_x_log10() + theme_classic() + ylab("Cell density") + geom_vline(xintercept = 500)

I'm wonder should the xintercept parameter changed by log10( )? Just as this : +geom_vline(xintercept = log10(500))

Thanks very much!

mistrm82 commented 1 month ago

Hi @chenqing1991 ,

It doesn't have to be on a log scale, but if you do then the differences are more emphasized