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hci-unihd / antibodies-analysis-issues

Issue tracker for problems in the antibodies analysis workflow.
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Partly out of focus - 20200417_152052_943 - A02-0005 #50

Open tischi opened 4 years ago

tischi commented 4 years ago

{"plateName":"20200417_152052_943","siteName":"A02-0005","pixelLocation":[5473.254599535554,1561.5105079537514,0.0]}

@imagirom @metavibor

Below image was correctly manually marked as invalid, I assume because a good fraction of it is out of focus.

I just wonder how this could be? Maybe some water drops at the plate bottom that produce some lens effect?

Screenshot

metavibor commented 4 years ago

@tischi we have a problem with the plate quality. All outer wells but especially A1-A5 and H1-H5 are so warped that in most fields of view it is not possible to make an in focus image across the entire field. There is a problem with plate supply, we had to use what was on the stock and we got these