Open vrbacky opened 5 years ago
Thanks for the bug report. The original error was caused by a warning message that was finding its way into the vcf output causing problems downstream. I have fixed this, but the problem remains that for some reason bscall is having problems finding both members of a pair. Is there any chance that you could share a part of your input bam file so that I can see what is happening?
You can send the file directly to my email address to avoid sharing it with everyone... (simon.heath@gmail.com)
Sure. Thank you very much. More by email.
Hi,
gemBS seems to be a very interesting toolkit. My wet lab colleagues use targeted approach - they amplify specific regions of bisulfite converted DNA and analyze it using amplicon sequening on Illumina MiSEQ. I came to their data rather by an accident because they were unable to use their methods.
I've tried to analyze their data using gemBS. I can map the data without any problem - paired end data are mapped to a sequence used as a reference. Almost all reads are mapped and are reported as correct pairs in gemBS mapping report. Samtools flagstat reports them as properly paired too. But I get a lot of warning and errors during methylation and variant calling. All reads are reported as follows in calling err file:
I can avoid the messages by changing
keep_improper_pairs
option toFalse
but I get all reads asPairNotFound
in calling json log.Is there any way how to analyze such data?
Thank you very much.
My config is: