Hello! I was able to generate a ks plot using wgd_dmd and wgd_ksd, but ran into some errors trying to run wgd_syn. A folder is still created with the following files: G_mac_hap1_CDS.fa.lists G_mac_hap1_CDS.fa_gene_order_perchrom.tsv families.tsv gene-table.csv iadhore.conf, but it looks like wgd_syn did not completely finish.
Here's stdout:
2024-08-23 12:50:50 INFO This is wgd v2.0.38 cli.py:34
INFO Checking cores and threads... core.py:35
INFO The number of logical CPUs/Hyper core.py:36
Threading in the system: 128
INFO The number of physical cores in the core.py:37
system: 64
INFO The number of actually usable CPUs in core.py:38
the system: 15
INFO Checking memory... core.py:40
INFO Total physical memory: 503.4771 GB core.py:41
INFO Available memory: 275.9073 GB core.py:42
INFO Free memory: 246.2832 GB core.py:43
2024-08-23 12:50:52 INFO Configuring I-ADHoRe co-linearity search cli.py:703
INFO Writing families file syn.py:96
INFO Writing gene lists syn.py:98
2024-08-23 12:50:53 INFO Writing config file syn.py:100
2024-08-23 12:50:54 INFO Running I-ADHoRe cli.py:707
and stderr:
Traceback (most recent call last):
File "/apps/eb/wgd/2.0.38-foss-2020b/bin/wgd", line 8, in <module>
sys.exit(cli())
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/click/core.py", line 829, in __call__
return self.main(*args, **kwargs)
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/click/core.py", line 782, in main
rv = self.invoke(ctx)
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/click/core.py", line 1259, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/click/core.py", line 1066, in invoke
return ctx.invoke(self.callback, **ctx.params)
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/click/core.py", line 610, in invoke
return callback(*args, **kwargs)
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/cli.py", line 669, in syn
_syn(**kwargs)
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/cli.py", line 708, in _syn
run_adhore(conf)
File "/apps/eb/wgd/2.0.38-foss-2020b/lib/python3.8/site-packages/wgd/syn.py", line 187, in run_adhore
completed = sp.run(['i-adhore', config_file], stderr=sp.PIPE, stdout=sp.PIPE)
File "/apps/eb/Python/3.8.6-GCCcore-10.2.0/lib/python3.8/subprocess.py", line 489, in run
with Popen(*popenargs, **kwargs) as process:
File "/apps/eb/Python/3.8.6-GCCcore-10.2.0/lib/python3.8/subprocess.py", line 854, in __init__
self._execute_child(args, executable, preexec_fn, close_fds,
File "/apps/eb/Python/3.8.6-GCCcore-10.2.0/lib/python3.8/subprocess.py", line 1702, in _execute_child
raise child_exception_type(errno_num, err_msg, err_filename)
FileNotFoundError: [Errno 2] No such file or directory: 'i-adhore'
Hello! I was able to generate a ks plot using wgd_dmd and wgd_ksd, but ran into some errors trying to run wgd_syn. A folder is still created with the following files: G_mac_hap1_CDS.fa.lists G_mac_hap1_CDS.fa_gene_order_perchrom.tsv families.tsv gene-table.csv iadhore.conf, but it looks like wgd_syn did not completely finish.
Here's stdout:
and stderr:
TIA for your help troubleshooting!