hellosunking / Msuite2

Msuite2: integrated DNA methylation data analysis toolkit with enhanced performance
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Errors in final steps of analysis #9

Closed mbassalbioinformatics closed 8 months ago

mbassalbioinformatics commented 11 months ago

Hi, using the latest version of msuite 2.2, there are errors that occur at the end of the analysis which prevent it from generating the report and finishing up correctly.

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/home/mbassal/tools/Msuite2/bin/merge.size.pl w per.chr/chr*.size
Use of uninitialized value in foreach loop entry at /home/mbassal/tools/Msuite2/bin/merge.size.pl line 65.
Use of uninitialized value in foreach loop entry at /home/mbassal/tools/Msuite2/bin/merge.size.pl line 65.
/home/mbassal/tools/Msuite2/bin/merge.size.pl c per.chr/rhr*.size
Use of uninitialized value in foreach loop entry at /home/mbassal/tools/Msuite2/bin/merge.size.pl line 65.
Use of uninitialized value in foreach loop entry at /home/mbassal/tools/Msuite2/bin/merge.size.pl line 65.
R --slave --args Msuite2.size Msuite2.w.size Msuite2.c.size 0 < /home/mbassal/tools/Msuite2/bin/plot.size.R
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R --slave --args Msuite2.lambda.size Msuite2.w.lambda.size Msuite2.c.lambda.size 0 < /home/mbassal/tools/Msuite2/bin/plot.size.R
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R --slave --args Msuite2.pUC19.size Msuite2.w.pUC19.size Msuite2.c.pUC19.size 0 < /home/mbassal/tools/Msuite2/bin/plot.size.R
Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec,  : 
  line 1 did not have 2 elements
Calls: read.table -> scan
Execution halted
make: *** [makefile:50: Msuite2.pUC19.size.pdf] Error 1
Error: it seems that the analysis has not finished yet.

Thoughts?

hellosunking commented 10 months ago

Hi,

Msuite v2.2 added support for pUC19 spike-in, which makes it incompatible with indices built for earlier versions. If you use pUC19 spike-in in your experiments, please rebuild the indices for Msuite2 v2.2, otherwiser you may down-grade to v2.1.

Hope this message solves your issue. Kun