1) spend more time on last 3 days and less on processing raw reads
differential isoform analysis?
minimum # reads per cell
umi-counting with kallisto how to aggregate umis from transcripts to genes
Practicals to add: slalom, RNA velocity, dropEST, UMI-tools, BEARscc, Libinorm, Cell-cycle analysis
Drop-seq pipeline
imputation: when is zero really a zero?
1) spend more time on last 3 days and less on processing raw reads differential isoform analysis? minimum # reads per cell umi-counting with kallisto how to aggregate umis from transcripts to genes Practicals to add: slalom, RNA velocity, dropEST, UMI-tools, BEARscc, Libinorm, Cell-cycle analysis Drop-seq pipeline imputation: when is zero really a zero?