hemberg-lab / scRNA.seq.course

Analysis of single cell RNA-seq data course

https://www.singlecellcourse.org

GNU General Public License v3.0

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Simulated vs Real data vs Integrated RNA seq data analysis #154

Closed AswinSSoman closed 2 years ago

AswinSSoman commented 5 years ago

1 What are the changes required if using sc-RNA seq count data simulated using popular methods like splatter compared to real data?

2 Methods to integrate different sc-RNA seq data sets?

3 Changes in analysis workflow when integrating sc-RNA seq data sets with each other and with other sc-omics data like ATAC-seq data?

wikiselev commented 5 years ago

Many thanks! These are very good question, hopefully will be answered in the updated version of the course. At the moment, it is quite outdated, because we didn't have time to work on it.

AswinSSoman commented 5 years ago

Thanks for the update.

But I don't think the course is outdated, since it was last updated on 2018-05-29, by the time several more better alternatives for multiple steps in sc-RNA seq data analysis were already published. This is a great work! Would you mind adding more updates to this course so it is clear how many alternatives are there and which would be ideal for different cases?

Best regards, Aswin