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hemberg-lab / scRNA.seq.course

Analysis of single cell RNA-seq data course
https://www.singlecellcourse.org
GNU General Public License v3.0
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function isSpike is deprecated #171

Closed dianaliduke closed 4 years ago

dianaliduke commented 4 years ago

Hi all, your code including isSpike is not working correctly, because isSpike is now a deprecated function. Please advise on how to proceed.

Screen Shot 2020-05-15 at 11 44 20 AM
yachenshen2017 commented 4 years ago

Hi, Did you find the way to instead of isSpike in R? I have the same problem. Thanks

dianaliduke commented 4 years ago

Hi, I did not. I am trying to learn how to analyze data from drop-seq single cell RNA sequencing, and I realized that I do not need the isSpike function or the newer AltExp function to analyze data from drop-seq.

Someone answered my same question on bioinformatics stack exchange though. please follow the link here.

https://bioinformatics.stackexchange.com/questions/13295/isspike-function-in-singlecellexperiment-package-is-deprecated