Problems editing bases - Githubissues

hexylena / seqtrace

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Problems editing bases #4

Open GoogleCodeExporter opened 9 years ago

GoogleCodeExporter commented 9 years ago

Hello,

  I am running SeqTrace 0.9.0 using Ubuntu Linux v 13.1.0. I am using SeqTrace to view and edit ABI sequencer chromatogram files. However, every time I try to edit sequences, the menu buttons at the top of the sequence editing window are grayed out as are the editing options under the 'Edit' pull down menu. This happens when I open single or batch chromatogram files.  

  The sequencer machine used is an ABI 3130XL running KB Basecaller software v 1.4.0.

Any help you can provide will be greatly appreciated!

Thanks,
George

Original issue reported on code.google.com by g.hama...@gmail.com on 26 May 2014 at 2:08

GoogleCodeExporter commented 9 years ago

Hi, George,

Thanks for trying out SeqTrace and sending this issue report.

Just to make sure -- are you first using the mouse to select the bases you wish 
to edit (from the working/consensus sequence at the bottom of the trace view 
window)?  The editing controls will remain grayed out until one or more bases 
are selected.

Thanks,
Brian

Original comment by stucky.b...@gmail.com on 28 May 2014 at 6:47

GoogleCodeExporter commented 9 years ago

I think that I´m with the same problem.

Original comment by algas...@gmail.com on 5 Aug 2014 at 7:58

Attachments:

seq.png

GoogleCodeExporter commented 9 years ago

Dear Brian

now I understand how to use, I need select the base, not only click on.

Original comment by algas...@gmail.com on 5 Aug 2014 at 8:10

GoogleCodeExporter commented 9 years ago

I believe it should be easier to edit the sequences, because having to open and 
select an option for exchange takes too long. The rest of the software is great

Original comment by algas...@gmail.com on 5 Aug 2014 at 8:17

GoogleCodeExporter commented 9 years ago

I'm having the same issue using lubuntu 14.04. Even when I select/highlight a 
base, I'm still not able to edit. However, all of my confidence scores are 0, 
and I think this is probably the cause. Is there any way I can fix the 
confidence scores or tell seqtrace to ignore it (if my sequencing core doesn't 
actually include it)?

Original comment by chillywi...@gmail.com on 13 Oct 2014 at 6:23