Hi,
I tried to use lotus2 for my data (18s sequncese targeting protists,not bacteria and fungi) and I noticed that there are two options for the -tax_group .
My question is , what is the difference between the "bacteria|fungi" for tax_group ? Does -tax_group only influence annotation or also effect the upstream asv-generating steps?The results (asv/otu numbers and annotation results) using different tax_group settings seem different.
Hey,
the tax group is only used to guide automatic database choice, but if you define the database yourself this should not influence much, I think most of the parameters remain the same.
cheers, Falk
Hi, I tried to use lotus2 for my data (18s sequncese targeting protists,not bacteria and fungi) and I noticed that there are two options for the -tax_group .
My question is , what is the difference between the "bacteria|fungi" for tax_group ? Does -tax_group only influence annotation or also effect the upstream asv-generating steps?The results (asv/otu numbers and annotation results) using different tax_group settings seem different.