hildebra / lotus2

Amplicon sequencing pipelines suitable for SSU (16S, 18S), LSU (23S, 28S) and ITS.
http://lotus2.earlham.ac.uk/
GNU General Public License v3.0
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Analysis for protist #34

Closed liuchen92 closed 1 year ago

liuchen92 commented 1 year ago

Hi, I tried to use lotus2 for my data (18s sequncese targeting protists,not bacteria and fungi) and I noticed that there are two options for the -tax_group .

My question is , what is the difference between the "bacteria|fungi" for tax_group ? Does -tax_group only influence annotation or also effect the upstream asv-generating steps?The results (asv/otu numbers and annotation results) using different tax_group settings seem different.

hildebra commented 1 year ago

Hey, the tax group is only used to guide automatic database choice, but if you define the database yourself this should not influence much, I think most of the parameters remain the same. cheers, Falk