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hildebra / lotus2

Amplicon sequencing pipelines suitable for SSU (16S, 18S), LSU (23S, 28S) and ITS.
http://lotus2.earlham.ac.uk/
GNU General Public License v3.0
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parsing single read #42

Closed junhuili closed 1 year ago

junhuili commented 1 year ago

Hi Falk Hildebrand, I wonder if I can parse the single read sequences using lotus2? If so, how can I set the codes? Thanks, Junhui

hildebra commented 1 year ago

Hey Junhui, yes this is possible, e.g.as used for 454 / PacBio data. In the map, specifiy column "fastqFile" as per examples on the webpage, and simply give a single fastq file as input, instead of two read pairs. best, Falk

junhuili commented 1 year ago

Thanks, Falk.