error when using DADA2 - Githubissues

hildebra / lotus2

Amplicon sequencing pipelines suitable for SSU (16S, 18S), LSU (23S, 28S) and ITS.

http://lotus2.earlham.ac.uk/

GNU General Public License v3.0

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error when using DADA2 #58

Closed we32i225 closed 7 months ago

we32i225 commented 8 months ago

Hi, i have been using Lotus2 with no issue for some time now clustering via OTUs (standard 16s microbiome data). I have tried to change from UPARSE to DADA2. i have followed the suggestions on the lotus website but it doesn't seem to run. Is there a clear error in my input? (code below)

./lotus2 -i Will -o output_SLV_DADA2_2024 -m WillMAP.txt -refDB SLV -CL dada2 -taxAligner lambda

all help is appreciated -Will

we32i225 commented 8 months ago

sorry falk just realised i asked this a few months back haha

ozkurt commented 8 months ago

Hi Will,

Could you please share your log files with me?

Thanks, Ezgi