hillerlab / make_lastz_chains

Portable solution to generate genome alignment chains using lastz
MIT License
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Partial Lastz Process Timeouts in make_lastz_chains #43

Open ZhaoHang-bio opened 9 months ago

ZhaoHang-bio commented 9 months ago

Hi,

I've been utilizing the make_lastz_chains tool with the following command:

/public/home/zhaohang/soft/make_lastz_chains-2.0.7/make_chains.py target query ${refgenome_softmasked} ${quegenome_softmasked} --pd chain_out -f --chaining_memory 100 --cluster_executor slurm --cluster_queue smp01,amd,low --keep_temp I'm encountering an issue where some of the lastz subprocesses are timing out and failing to complete.

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Could this be due to the large size of the genome or the high proportion of repetitive sequences? What would be the recommended approach to mitigate this issue? Should I reduce the chunk size or extend the runtime of the subprocesses, and if so, could you provide guidance on how to adjust these parameters effectively?