Closed tpall closed 5 years ago
Building DAG of jobs... Using shell: /usr/bin/bash Provided cores: 2 Rules claiming more threads will be scaled down. Job counts: count jobs 1 megablast_nt 1 [Sat Jan 5 17:02:06 2019] rule megablast_nt: input: blast/SRR5580233_bac_unmapped_10_masked.fa, /gpfs/hpc/repository/VirusSeeker/databases/nt output: blast/SRR5580233_megablast_nt_10.tsv jobid: 0 wildcards: sample=SRR5580233, n=10 Activating conda environment: /gpfs/hpc/home/taavi74/fastq/prjna361402/.snakemake/conda/76feaf1a Traceback (most recent call last): File "/gpfs/hpc/home/taavi74/fastq/prjna361402/.snakemake/scripts/tmp2qetcyug.wrapper.py", line 30, in <module> run_blast(snakemake.input, snakemake.output, snakemake.params) File "/gpfs/hpc/home/taavi74/fastq/prjna361402/.snakemake/scripts/tmp2qetcyug.wrapper.py", line 27, in run_blast stdout, stderr = cline() File "/gpfs/hpc/home/taavi74/fastq/prjna361402/.snakemake/conda/76feaf1a/lib/python3.6/site-packages/Bio/Application/__init__.py", line 523, in __call__ stdout_str, stderr_str) Bio.Application.ApplicationError: Non-zero return code 2 from "blastn -out blast/SRR5580233_megablast_nt_10.tsv -outfmt '6 qseqid sgi pident length mismatch gapopen qstart qend sstart send evalue bitscore' -show_gis -query blast/SRR5580233_bac_unmapped_10_masked.fa -db /gpfs/hpc/repository/VirusSeeker/databases/nt -evalue 1e-8 -word_size 16 -max_hsps 50 -num_threads 8 -task megablast", message 'BLAST Database error: No alias or index file found for nucleotide database [/gpfs/hpc/repository/VirusSeeker/databases/nt] in search path [/gpfs/hpc/home/taavi74/fastq/prjna361402::]' [Sat Jan 5 17:02:07 2019] Error in rule megablast_nt: jobid: 0 output: blast/SRR5580233_megablast_nt_10.tsv conda-env: /gpfs/hpc/home/taavi74/fastq/prjna361402/.snakemake/conda/76feaf1a RuleException: CalledProcessError in line 164 of /gpfs/hpc/home/taavi74/Projects/vs/rules/blast.smk:
tpall
commented
5 years ago