I installed CellNeighborEx according the specs. Downloaded the tutorial data sets and ran the notebooks. Only tutorial_SSV2embryo runs till completion. The other notebooks all gave the same error:
Running the cell with the code:
# If save=True, all result files (DEG list: csv, heatmaps and volcano plots: pdf, gene expression values: txt) are saved in the "DE_results" folder in the root directory.
DEG_list = CellNeighborEX.DEanalysis.analyze_data(df_cell_id, df_gene_name, df_log_data, path_categorization, data_type, lrCutoff, pCutoff, pCutoff2, direction, normality_test, top_genes, save=True)
The error generated looks like this:
IndexError Traceback (most recent call last)
Cell In[22], line 2
1 # If save=True, all result files (DEG list: csv, heatmaps and volcano plots: pdf, gene expression values: txt) are saved in the "DE_results" folder in the root directory.
----> 2 DEG_list = CellNeighborEX.DEanalysis.analyze_data(df_cell_id, df_gene_name, df_log_data, path_categorization, data_type, lrCutoff, pCutoff, pCutoff2, direction, normality_test, top_genes, save=True)
File ~/anaconda3/envs/cellneighborex/lib/python3.10/site-packages/CellNeighborEX/DEanalysis.py:1125, in analyze_data(df_cell_id, df_gene_name, df_log_data, path_categorization, data_type, lrCutoff, pCutoff, pCutoff2, direction, normality_test, top_genes, save, root)
1122 cell_id = cell_id.reset_index()
1124 log_data_total = log_data
-> 1125 log_data = log_data_total.iloc[:, selected_row.index]
1126 column_names = [str(i) for i in range(len(matchComb))]
1127 log_data.columns = column_names
File ~/.local/lib/python3.10/site-packages/pandas/core/indexing.py:1067, in _LocationIndexer.__getitem__(self, key)
1065 if self._is_scalar_access(key):
1066 return self.obj._get_value(*key, takeable=self._takeable)
-> 1067 return self._getitem_tuple(key)
1068 else:
1069 # we by definition only have the 0th axis
1070 axis = self.axis or 0
File ~/.local/lib/python3.10/site-packages/pandas/core/indexing.py:1563, in _iLocIndexer._getitem_tuple(self, tup)
1561 def _getitem_tuple(self, tup: tuple):
-> 1563 tup = self._validate_tuple_indexer(tup)
1564 with suppress(IndexingError):
1565 return self._getitem_lowerdim(tup)
File ~/.local/lib/python3.10/site-packages/pandas/core/indexing.py:873, in _LocationIndexer._validate_tuple_indexer(self, key)
871 for i, k in enumerate(key):
872 try:
--> 873 self._validate_key(k, i)
874 except ValueError as err:
875 raise ValueError(
876 "Location based indexing can only have "
877 f"[{self._valid_types}] types"
878 ) from err
File ~/.local/lib/python3.10/site-packages/pandas/core/indexing.py:1481, in _iLocIndexer._validate_key(self, key, axis)
1479 # check that the key does not exceed the maximum size of the index
1480 if len(arr) and (arr.max() >= len_axis or arr.min() < -len_axis):
-> 1481 raise IndexError("positional indexers are out-of-bounds")
1482 else:
1483 raise ValueError(f"Can only index by location with a [{self._valid_types}]")
IndexError: positional indexers are out-of-bounds
I installed CellNeighborEx according the specs. Downloaded the tutorial data sets and ran the notebooks. Only tutorial_SSV2embryo runs till completion. The other notebooks all gave the same error: Running the cell with the code:
The error generated looks like this: