Overview:
The Frequency plot is showing incorrect cluster count data when the 'complement' button has been used in the Data Exploration module.
To reproduce it:
-In data exploration, create a couple of custom cell sets. In the example, I created 3.
-Select all the custom cell sets and create a complement of the selected sets. In the example, I named these ‘unassigned’.
-Note that the number of unassigned cells is reported correctly in the Data Exploration module. In the example, it's 5203 cells in the unassigned cluster across all samples.
-Then go to the Frequency plot in Plots and Tables module. Select to view the custom cell sets on the plot, and select 'count'. You can see that the number of cells in the unassigned cluster is much more than 5203!
To do:
Fix the bug so that the frequency plot is showing the correct number of cells.
Check other functionality in the platform where cell number is reported to check that it's displaying the correct number of cells in the unassigned cluster of the custom cell sets. E.g. download the CSV file from the frequency plot, and download the expression matrix.
The exptID that I used as an example in the Biomage instance is: 16bd43ff-1f0b-4b9b-b94b-407ce5137b61 (owned by Vicky)
Overview: The Frequency plot is showing incorrect cluster count data when the 'complement' button has been used in the Data Exploration module.
To reproduce it: -In data exploration, create a couple of custom cell sets. In the example, I created 3. -Select all the custom cell sets and create a complement of the selected sets. In the example, I named these ‘unassigned’. -Note that the number of unassigned cells is reported correctly in the Data Exploration module. In the example, it's 5203 cells in the unassigned cluster across all samples. -Then go to the Frequency plot in Plots and Tables module. Select to view the custom cell sets on the plot, and select 'count'. You can see that the number of cells in the unassigned cluster is much more than 5203!
To do: Fix the bug so that the frequency plot is showing the correct number of cells. Check other functionality in the platform where cell number is reported to check that it's displaying the correct number of cells in the unassigned cluster of the custom cell sets. E.g. download the CSV file from the frequency plot, and download the expression matrix.
The exptID that I used as an example in the Biomage instance is: 16bd43ff-1f0b-4b9b-b94b-407ce5137b61 (owned by Vicky)