hms-dbmi / dseqr

single-cell and bulk RNA-seq analyses from counts → pathways → drug candidates.
https://docs.dseqr.com
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permit exploring original labels after single cell sample integration #135

Closed alexvpickering closed 4 years ago

alexvpickering commented 4 years ago

After sample integration there are often cells from one sample that and inappropriately clustered with cells from another sample. In my experience this has generally been when a cell type is exclusive to one sample.

The best way that I have found to resolve this is to identify it and then redo the integration excluding the exclusive cluster. Clusters can currently be excluded during integration. This would permit identifying when the issue has occurred.