Without using group argument in edgeR::filterByExpr can get inappropriate filtering. For example ACE2 get's filtered in GSE147507 as it is only expressed in one of the cell types tested. This behaviour is resolved in filtering is delayed until after groups have been annotated.
Without using
group
argument inedgeR::filterByExpr
can get inappropriate filtering. For example ACE2 get's filtered in GSE147507 as it is only expressed in one of the cell types tested. This behaviour is resolved in filtering is delayed until after groups have been annotated.