in our sequencing, we see that a single, highly expressed marker gene is the main driver of overdispersion in many pathways related to it. It seems that the marker gene is expressed lower in a subpopulation of the cells, but still at a high level. I tried to subtract the aspects of those pathways, but they are still overdispersed after pagoda.subtract.aspect. Is there a way to explicitly account for the variability of a single gene?
jenzopr
commented
8 years ago
Hi Jean-Fan,
in our sequencing, we see that a single, highly expressed marker gene is the main driver of overdispersion in many pathways related to it. It seems that the marker gene is expressed lower in a subpopulation of the cells, but still at a high level. I tried to subtract the aspects of those pathways, but they are still overdispersed after pagoda.subtract.aspect. Is there a way to explicitly account for the variability of a single gene?
Best, Jens