For Express Compare, it would be really helpful to assess the efficacy of Poly(A)+ and RiboFree in either not capturing, or eliminating rRNA. Both NCBI and the Rfam databases allow FASTA file download of 25S, 18S and 5S rRNA sequences which I would then create a Custom BLAST database from the sequences and BLAST against the db. On both databases I searched for Montipora capitata, Pocillopora acuta, and Aiptasia rRNA sequences with 0 results each time.
For Express Compare, it would be really helpful to assess the efficacy of Poly(A)+ and RiboFree in either not capturing, or eliminating rRNA. Both NCBI and the Rfam databases allow FASTA file download of 25S, 18S and 5S rRNA sequences which I would then create a Custom BLAST database from the sequences and BLAST against the db. On both databases I searched for Montipora capitata, Pocillopora acuta, and Aiptasia rRNA sequences with 0 results each time.